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Application of Sephadex LH-20 for Microdetermination of Dopamine by Solid Phase Spectrophotometry
Joint Authors
Mohamadipour-taziyan, Arash
Taghdiri, Mehdi
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-28
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Pharmacy, Health & Medical Sciences
Pharmacology
Abstract EN
A sensitive spectrophotometric method for the determination of dopamine was carried out without any separation steps.
Bromocresol green is adsorbed on Sephadex LH-20 gel but the sorption decreases in the presence of dopamine due to ion-pair formation between bromocresol green and dopamine in solution.
This attenuation was used to the microdetermination of dopamine by measurement of absorbance of the solid phase (Sephadex LH-20 gel) in a 1.0 mm cell at 625 nm.
Dopamine could be determined in the concentration range of 0.4–1.6 μg mL−1 (10-mL Sample volume) with a relative standard deviation (RSD) of 0.03% (n=4).
The detection limit was obtained, 0.26 μg mL−1 (1.7 μM).
The method was used for determination of dopamine in pharmaceutical injection sample and satisfactory result was obtained.
American Psychological Association (APA)
Taghdiri, Mehdi& Mohamadipour-taziyan, Arash. 2012. Application of Sephadex LH-20 for Microdetermination of Dopamine by Solid Phase Spectrophotometry. ISRN Pharmaceutics،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-455271
Modern Language Association (MLA)
Taghdiri, Mehdi& Mohamadipour-taziyan, Arash. Application of Sephadex LH-20 for Microdetermination of Dopamine by Solid Phase Spectrophotometry. ISRN Pharmaceutics No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-455271
American Medical Association (AMA)
Taghdiri, Mehdi& Mohamadipour-taziyan, Arash. Application of Sephadex LH-20 for Microdetermination of Dopamine by Solid Phase Spectrophotometry. ISRN Pharmaceutics. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-455271
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-455271