ATP Is Required and Advances Cytokine-Induced Gap Junction Formation in Microglia In Vitro
Joint Authors
Retamal, Mauricio A.
von Bernhardi, Rommy
Shoji, Kenji F.
Jiang, Jean X.
Harcha, Paloma A.
Ramírez, Gigliola
Sáez, Pablo J.
Sáez, Juan C.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-23
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Microglia are the immune cells in the central nervous system.
After injury microglia release bioactive molecules, including cytokines and ATP, which modify the functional state of hemichannels (HCs) and gap junction channels (GJCs), affecting the intercellular communication via extracellular and intracellular compartments, respectively.
Here, we studied the role of extracellular ATP and several cytokines as modulators of the functional state of microglial HCs and GJCs using dye uptake and dye coupling techniques, respectively.
In microglia and the microglia cell line EOC20, ATP advanced the TNF-α/IFN-γ-induced dye coupling, probably through the induction of IL-1β release.
Moreover, TNF-α/IFN-γ, but not TNF-α plus ATP, increased dye uptake in EOC20 cells.
Blockade of Cx43 and Panx1 HCs prevented dye coupling induced by TNF-α/IFN-γ, but not TNF-α plus ATP.
In addition, IL-6 prevented the induction of dye coupling and HC activity induced by TNF-α/IFN-γ in EOC20 cells.
Our data support the notion that extracellular ATP affects the cellular communication between microglia through autocrine and paracrine mechanisms, which might affect the timing of immune response under neuroinflammatory conditions.
American Psychological Association (APA)
Sáez, Pablo J.& Shoji, Kenji F.& Retamal, Mauricio A.& Harcha, Paloma A.& Ramírez, Gigliola& Jiang, Jean X.…[et al.]. 2013. ATP Is Required and Advances Cytokine-Induced Gap Junction Formation in Microglia In Vitro. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-16.
https://search.emarefa.net/detail/BIM-455306
Modern Language Association (MLA)
Sáez, Pablo J.…[et al.]. ATP Is Required and Advances Cytokine-Induced Gap Junction Formation in Microglia In Vitro. Mediators of Inflammation No. 2013 (2013), pp.1-16.
https://search.emarefa.net/detail/BIM-455306
American Medical Association (AMA)
Sáez, Pablo J.& Shoji, Kenji F.& Retamal, Mauricio A.& Harcha, Paloma A.& Ramírez, Gigliola& Jiang, Jean X.…[et al.]. ATP Is Required and Advances Cytokine-Induced Gap Junction Formation in Microglia In Vitro. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-16.
https://search.emarefa.net/detail/BIM-455306
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-455306