Assessment of the Morphological, Biochemical, and Kinetic Properties for Candida rugosa Lipase Immobilized on Hydrous Niobium Oxide to Be Used in the Biodiesel Synthesis
Joint Authors
de Castro, Heizir F.
Miranda, Michele
Andrade Souza, Livia T.
Urioste, Daniele
Mendes, Adriano A.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-16
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Lipase from Candida rugosa (CRL) was immobilized by covalent attachment on hydrous niobium oxide.
The matrix could effectively be attached to the enzyme with high retention of activity and prevent its leakage.
Following immobilization, CRL exhibited improved storage stability and performed better at higher incubation temperatures.
In addition, the enzyme retained most of its catalytic efficiency after successive operational cycles.
The immobilized derivative was also fully characterized with respect to its morphological properties: particle size, surface specific area, and pore size distribution.
Structural integrity and conformational changes, such as surface cavities in the support, set by the lipase procedure, were observed by Scanning Electron Microscopy.
Additionally, a comparative study between free and immobilized lipases was provided in terms of pH, temperature, and thermal stability.
CRL derivative was evaluated for the synthesis of biodiesel employing babassu oil and short chain alcohols.
The process was feasible only for oil and butanol reaction system.
American Psychological Association (APA)
Miranda, Michele& Urioste, Daniele& Andrade Souza, Livia T.& Mendes, Adriano A.& de Castro, Heizir F.. 2011. Assessment of the Morphological, Biochemical, and Kinetic Properties for Candida rugosa Lipase Immobilized on Hydrous Niobium Oxide to Be Used in the Biodiesel Synthesis. Enzyme Research،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-455311
Modern Language Association (MLA)
Miranda, Michele…[et al.]. Assessment of the Morphological, Biochemical, and Kinetic Properties for Candida rugosa Lipase Immobilized on Hydrous Niobium Oxide to Be Used in the Biodiesel Synthesis. Enzyme Research No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-455311
American Medical Association (AMA)
Miranda, Michele& Urioste, Daniele& Andrade Souza, Livia T.& Mendes, Adriano A.& de Castro, Heizir F.. Assessment of the Morphological, Biochemical, and Kinetic Properties for Candida rugosa Lipase Immobilized on Hydrous Niobium Oxide to Be Used in the Biodiesel Synthesis. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-455311
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-455311