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Cow Dung Substrate for the Potential Production of Alkaline Proteases by Pseudomonas putida Strain AT in Solid-State Fermentation
Joint Authors
Vijayaraghavan, Ponnuswamy
Saranya, Sreekumar
Prakash Vincent, Samuel Gnana
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-01
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Cow dung and agroresidues were used as the substrates for the production of alkaline proteases by Pseudomonas putida strain AT in solid-state fermentation.
Among the various substrates evaluated, cow dung supported maximum (1351±217 U/g) protease production.
The optimum conditions for the production of alkaline proteases were a fermentation period of 48 h, 120% (v/w) moisture, pH 9, and the addition of 6% (v/w) inoculum, 1.5% (w/w) trehalose, and 2.0% (w/w) yeast extract to the cow dung substrate.
The enzyme was active over a range of temperatures (50–70°C) and pHs (8–10), with maximum activity at 60°C and pH 9.
These enzymes showed stability towards surfactants, detergents, and solvent and digested various natural proteins.
American Psychological Association (APA)
Vijayaraghavan, Ponnuswamy& Saranya, Sreekumar& Prakash Vincent, Samuel Gnana. 2014. Cow Dung Substrate for the Potential Production of Alkaline Proteases by Pseudomonas putida Strain AT in Solid-State Fermentation. Chinese Journal of Biology،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-455399
Modern Language Association (MLA)
Vijayaraghavan, Ponnuswamy…[et al.]. Cow Dung Substrate for the Potential Production of Alkaline Proteases by Pseudomonas putida Strain AT in Solid-State Fermentation. Chinese Journal of Biology No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-455399
American Medical Association (AMA)
Vijayaraghavan, Ponnuswamy& Saranya, Sreekumar& Prakash Vincent, Samuel Gnana. Cow Dung Substrate for the Potential Production of Alkaline Proteases by Pseudomonas putida Strain AT in Solid-State Fermentation. Chinese Journal of Biology. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-455399
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-455399