Optimization of Aqueous Two-Phase Systems for the Recovery of Soluble Proteins from Tannery Wastewater Using Response Surface Methodology
Joint Authors
Murty, Vytla Ramachandra
Raja, Selvaraj
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-12
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Aqueous two-phase system (ATPS) composed of polyethylene glycol 6000 (PEG 6000) and sodium citrate (SC) has been proposed to recover the valuable soluble proteins from tannery wastewater.
A sequential optimization strategy which included fractional factorial design (fFD) and central composite design (CCD) was employed to enhance the recovery.
From this strategy, a second-order polynomial model was obtained for the protein recovery and it was validated.
The optimum recovery was found as 93.46% when pH, NaCl concentration, and temperature were kept at 7.5, 0.1 M, and 33°C, respectively, for a phase system composed of 20% (w/w) PEG 6000-15% (w/w) SC.
Thus the proposed ATPS can serve as an alternative to the conventional precipitation method to recover the soluble proteins from tannery wastewater.
American Psychological Association (APA)
Raja, Selvaraj& Murty, Vytla Ramachandra. 2013. Optimization of Aqueous Two-Phase Systems for the Recovery of Soluble Proteins from Tannery Wastewater Using Response Surface Methodology. Journal of Engineering،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-455404
Modern Language Association (MLA)
Raja, Selvaraj& Murty, Vytla Ramachandra. Optimization of Aqueous Two-Phase Systems for the Recovery of Soluble Proteins from Tannery Wastewater Using Response Surface Methodology. Journal of Engineering No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-455404
American Medical Association (AMA)
Raja, Selvaraj& Murty, Vytla Ramachandra. Optimization of Aqueous Two-Phase Systems for the Recovery of Soluble Proteins from Tannery Wastewater Using Response Surface Methodology. Journal of Engineering. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-455404
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-455404