Centrosome Aberrations Associated with Cellular Senescence and p53 Localization at Supernumerary Centrosomes
Author
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-03
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Centrosome overduplication or amplification has been observed in many human cancers and in premalignant tissue, but the mechanisms leading to such centrosome aberrations are not fully understood.
We previously showed that abnormal mitotic cells with supernumerary centrosomes increase with replicative senescence in human fibroblasts, especially in a polyploid subpopulation.
This study examines localization of p53 protein at centrosomes in mitotic cells, which is often observed in association with DNA damage response, to investigate a possible association between p53 localization and numerical centrosome aberrations induced by cellular senescence.
Cultures at later passages or the 4th day after exposure to H2O2 showed increased frequencies of mitotic cells with supernumerary centrosomes, especially in a polyploid subpopulation.
Immunohistochemical analysis frequently showed p53-positive foci in mitotic cells, and some were localized at centrosomes.
The number of p53-positive foci in mitotic cells and its localization to centrosomes increased with replicative and premature senescence.
Supernumerary centrosomes showed higher frequencies of p53 localization compared to normally duplicated centrosomes.
Centrosome-associated p53 protein was phosphorylated at Ser15.
These data suggest a possible association between localization of p53 protein and numerical centrosome aberrations in replicatively or prematurely senescent cells.
American Psychological Association (APA)
Ohshima, Susumu. 2012. Centrosome Aberrations Associated with Cellular Senescence and p53 Localization at Supernumerary Centrosomes. Oxidative Medicine and Cellular Longevity،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-455422
Modern Language Association (MLA)
Ohshima, Susumu. Centrosome Aberrations Associated with Cellular Senescence and p53 Localization at Supernumerary Centrosomes. Oxidative Medicine and Cellular Longevity No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-455422
American Medical Association (AMA)
Ohshima, Susumu. Centrosome Aberrations Associated with Cellular Senescence and p53 Localization at Supernumerary Centrosomes. Oxidative Medicine and Cellular Longevity. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-455422
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-455422