Cellular Redox Status Regulates Emodin-Induced Radiosensitization of Nasopharyngeal Carcinoma Cells In Vitro and In Vivo
Joint Authors
Zhou, Yi
Hou, Huaxin
Cheng, Daohai
Li, Danrong
Liu, Ying
Li, Li
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Pharmacy, Health & Medical Sciences
Economics & Business Administration
Pharmacology
Abstract EN
Here, we report that regulation of cellular redox status is required for radiosensitization of nasopharyngeal carcinoma (NPC) cells by emodin.
We evaluated emodin’s radiosensitivity-enhancing ability by using NPC cells in vitro and xenografts in vivo.
A clonogenic assay was performed to evaluate NPC cell survival and to determine dose modification factors.
Flow cytometry, western blot analysis, and in vivo radiation-induced tumor regrowth delay assays were performed to characterize emodin’s effects.
Exposure of CNE-1 NPC cells to emodin enhanced their radiosensitivity.
HIF-1α expression significantly increased under hypoxic conditions but did not change after treatment with emodin alone.
Emodin downregulated mRNA and protein expression of HIF-1α.
Cells exposed to radiation and emodin underwent significant cell cycle arrest at the G2/M phase.
The percentage of apoptotic cells and reactive oxygen species (ROS) levels were significantly higher in the group exposed to emodin and radiation hypoxic group than in the other groups.
Compared to the CNE-1 xenografts exposed to radiation alone, CNE-1 xenografts exposed to radiation with emodin showed significantly enhanced radiation effects.
Our data suggest that emodin effectively enhanced the radiosensitivity of CNE-1 cells in vitro and in vivo.
The mechanism appears to involve ROS generation and ROS-mediated inhibition of HIF-1α expression.
American Psychological Association (APA)
Hou, Huaxin& Li, Danrong& Cheng, Daohai& Li, Li& Liu, Ying& Zhou, Yi. 2013. Cellular Redox Status Regulates Emodin-Induced Radiosensitization of Nasopharyngeal Carcinoma Cells In Vitro and In Vivo. Journal of Pharmaceutics،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-455481
Modern Language Association (MLA)
Hou, Huaxin…[et al.]. Cellular Redox Status Regulates Emodin-Induced Radiosensitization of Nasopharyngeal Carcinoma Cells In Vitro and In Vivo. Journal of Pharmaceutics No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-455481
American Medical Association (AMA)
Hou, Huaxin& Li, Danrong& Cheng, Daohai& Li, Li& Liu, Ying& Zhou, Yi. Cellular Redox Status Regulates Emodin-Induced Radiosensitization of Nasopharyngeal Carcinoma Cells In Vitro and In Vivo. Journal of Pharmaceutics. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-455481
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-455481