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Intracellular Delivery of siRNA by Polycationic Superparamagnetic Nanoparticles
Joint Authors
González, Carlos I.
Bromberg, Lev
González Feliciano, Jose A.
Hatton, T. Alan
Barletta, Gabriel
Badillo, Valerie
Castillo, Betzaida
López, Xaira
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-08-30
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The siRNA transfection efficiency of nanoparticles (NPs), composed of a superparamagnetic iron oxide core modified with polycationic polymers (poly(hexamethylene biguanide) or branched polyethyleneimine), were studied in CHO-K1 and HeLa cell lines.
Both NPs demonstrated to be good siRNA transfection vehicles, but unmodified branched polyethyleneimine (25 kD) was superior on both cell lines.
However, application of an external magnetic field during transfection (magnetofection) increased the efficiency of the superparamagnetic NPs.
Furthermore, our results reveal that these NPs are less toxic towards CHO-K1 cell lines than the unmodified polycationic-branched polyethyleneimine (PEI).
In general, the external magnetic field did not alter the cell’s viability nor it disrupted the cell membranes, except for the poly(hexamethylene biguanide)-modified NP, where it was observed that in CHO-K1 cells application of the external magnetic field promoted membrane damage.
This paper presents new polycationic superparamagnetic NPs as promising transfection vehicles for siRNA and demonstrates the advantages of magnetofection.
American Psychological Association (APA)
Castillo, Betzaida& Bromberg, Lev& López, Xaira& Badillo, Valerie& González Feliciano, Jose A.& González, Carlos I.…[et al.]. 2012. Intracellular Delivery of siRNA by Polycationic Superparamagnetic Nanoparticles. Journal of Drug Delivery،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-455543
Modern Language Association (MLA)
Castillo, Betzaida…[et al.]. Intracellular Delivery of siRNA by Polycationic Superparamagnetic Nanoparticles. Journal of Drug Delivery No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-455543
American Medical Association (AMA)
Castillo, Betzaida& Bromberg, Lev& López, Xaira& Badillo, Valerie& González Feliciano, Jose A.& González, Carlos I.…[et al.]. Intracellular Delivery of siRNA by Polycationic Superparamagnetic Nanoparticles. Journal of Drug Delivery. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-455543
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-455543