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Adaptation of Escherichia coli ATCC 8739 to 11% NaCl
Joint Authors
Lim, Joshua Z. R.
How, Jian Ann
Lee, Chin How
Goh, Desmond J. W.
Ling, Maurice H. T.
Ng, Wei Chuan
Oon, Jack S. H.
Lee, Kun Cheng
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-13
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Escherichia coli (E.
coli) is a nonhalophilic microbe and used to indicate faecal contamination.
Salt (sodium chloride, NaCl) is a common food additive and is used in preservatives to encounter microbial growth.
The effect of how E.
coli interacts with the salt present in the human diet is unclear.
Thus, it is important to investigate this relationship.
In order to adapt and survive the changes in the environment, E.
coli may undergo halophilization.
In this study, we observed the genetic changes and growth kinetics of E.
coli ATCC 8739 under 3%–11% NaCl over 80 passages.
Our results suggest that E.
coli adapted to 1% increase in NaCl every month with a successful adaptation to 11% NaCl.
Gram staining and PCR/RFLP showed that the cultures are Gram negative and the DNA profiles of all 4 replicates to be similar, suggesting that the cultures had not been contaminated.
American Psychological Association (APA)
How, Jian Ann& Lim, Joshua Z. R.& Goh, Desmond J. W.& Ng, Wei Chuan& Oon, Jack S. H.& Lee, Kun Cheng…[et al.]. 2012. Adaptation of Escherichia coli ATCC 8739 to 11% NaCl. Dataset Papers in Biology،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-455559
Modern Language Association (MLA)
How, Jian Ann…[et al.]. Adaptation of Escherichia coli ATCC 8739 to 11% NaCl. Dataset Papers in Biology No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-455559
American Medical Association (AMA)
How, Jian Ann& Lim, Joshua Z. R.& Goh, Desmond J. W.& Ng, Wei Chuan& Oon, Jack S. H.& Lee, Kun Cheng…[et al.]. Adaptation of Escherichia coli ATCC 8739 to 11% NaCl. Dataset Papers in Biology. 2012. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-455559
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-455559