Antimicrobial Activity of Cladonia verticillaris Lichen Preparations on Bacteria and Fungi of Medical Importance

Joint Authors

Barroso Coelho, Luana Cassandra Breitenbach
Gomes, Francis Soares
da Silva, Michele Dalvina Correia
Paiva, Patrícia Maria Guedes
Napoleão, Thiago Henrique
Ramos, Dalila de Brito Marques

Source

Chinese Journal of Biology

Issue

Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2014-01-22

Country of Publication

Egypt

No. of Pages

7

Main Subjects

Biology

Abstract EN

Cladonia verticillaris lichen lectin (ClaveLL) was purified using a previously established protocol and then evaluated for its potential antimicrobial activity.

Initially, the autochthonous lichen was submitted to extraction with sodium phosphate buffer pH 7.0, followed by filtration and centrifugation to obtain crude extract.

A salt fractionation was performed with 30% ammonium sulfate.

After centrifugation, the protein fraction was loaded onto molecular exclusion chromatography using Sephadex G-100 matrix to purify active lectin.

ClaveLL showed antibacterial activity against Gram-positive (Bacillus subtilis, Staphylococcus aureus, and Enterococcus faecalis) and Gram-negative (Escherichia coli and Klebsiella pneumoniae) assayed strains, with greater inhibitory effect on growth of E.

coli (MIC of 7.18 μg mL−1).

The lowest minimum bactericidal concentration (MBC, 57.4 μg mL−1) was detected against E.

faecalis.

The antifungal assay performed with Trichophyton mentagrophytes, Microsporum gypseum, Trichophyton rubrum, Trichosporon cutaneum, and Trichosporon asahi evaluated crude extract, fraction, and ClaveLL preparations.

ClaveLL was the most active against T.

rubrum with an inhibition percentage of 35% compared to negative control (phosphate buffer).

Extract and fraction showed better activity on growth inhibition of T.

mentagrophytes (35%).

The results indicate the potential of ClaveLL and other C.

verticillaris preparations as antimicrobial agents useful for applications focusing on human health.

American Psychological Association (APA)

Ramos, Dalila de Brito Marques& Gomes, Francis Soares& Napoleão, Thiago Henrique& Paiva, Patrícia Maria Guedes& da Silva, Michele Dalvina Correia& Barroso Coelho, Luana Cassandra Breitenbach. 2014. Antimicrobial Activity of Cladonia verticillaris Lichen Preparations on Bacteria and Fungi of Medical Importance. Chinese Journal of Biology،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-455596

Modern Language Association (MLA)

Ramos, Dalila de Brito Marques…[et al.]. Antimicrobial Activity of Cladonia verticillaris Lichen Preparations on Bacteria and Fungi of Medical Importance. Chinese Journal of Biology No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-455596

American Medical Association (AMA)

Ramos, Dalila de Brito Marques& Gomes, Francis Soares& Napoleão, Thiago Henrique& Paiva, Patrícia Maria Guedes& da Silva, Michele Dalvina Correia& Barroso Coelho, Luana Cassandra Breitenbach. Antimicrobial Activity of Cladonia verticillaris Lichen Preparations on Bacteria and Fungi of Medical Importance. Chinese Journal of Biology. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-455596

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-455596