Antimicrobial Activity of Cladonia verticillaris Lichen Preparations on Bacteria and Fungi of Medical Importance
Joint Authors
Barroso Coelho, Luana Cassandra Breitenbach
Gomes, Francis Soares
da Silva, Michele Dalvina Correia
Paiva, Patrícia Maria Guedes
Napoleão, Thiago Henrique
Ramos, Dalila de Brito Marques
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-22
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Cladonia verticillaris lichen lectin (ClaveLL) was purified using a previously established protocol and then evaluated for its potential antimicrobial activity.
Initially, the autochthonous lichen was submitted to extraction with sodium phosphate buffer pH 7.0, followed by filtration and centrifugation to obtain crude extract.
A salt fractionation was performed with 30% ammonium sulfate.
After centrifugation, the protein fraction was loaded onto molecular exclusion chromatography using Sephadex G-100 matrix to purify active lectin.
ClaveLL showed antibacterial activity against Gram-positive (Bacillus subtilis, Staphylococcus aureus, and Enterococcus faecalis) and Gram-negative (Escherichia coli and Klebsiella pneumoniae) assayed strains, with greater inhibitory effect on growth of E.
coli (MIC of 7.18 μg mL−1).
The lowest minimum bactericidal concentration (MBC, 57.4 μg mL−1) was detected against E.
faecalis.
The antifungal assay performed with Trichophyton mentagrophytes, Microsporum gypseum, Trichophyton rubrum, Trichosporon cutaneum, and Trichosporon asahi evaluated crude extract, fraction, and ClaveLL preparations.
ClaveLL was the most active against T.
rubrum with an inhibition percentage of 35% compared to negative control (phosphate buffer).
Extract and fraction showed better activity on growth inhibition of T.
mentagrophytes (35%).
The results indicate the potential of ClaveLL and other C.
verticillaris preparations as antimicrobial agents useful for applications focusing on human health.
American Psychological Association (APA)
Ramos, Dalila de Brito Marques& Gomes, Francis Soares& Napoleão, Thiago Henrique& Paiva, Patrícia Maria Guedes& da Silva, Michele Dalvina Correia& Barroso Coelho, Luana Cassandra Breitenbach. 2014. Antimicrobial Activity of Cladonia verticillaris Lichen Preparations on Bacteria and Fungi of Medical Importance. Chinese Journal of Biology،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-455596
Modern Language Association (MLA)
Ramos, Dalila de Brito Marques…[et al.]. Antimicrobial Activity of Cladonia verticillaris Lichen Preparations on Bacteria and Fungi of Medical Importance. Chinese Journal of Biology No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-455596
American Medical Association (AMA)
Ramos, Dalila de Brito Marques& Gomes, Francis Soares& Napoleão, Thiago Henrique& Paiva, Patrícia Maria Guedes& da Silva, Michele Dalvina Correia& Barroso Coelho, Luana Cassandra Breitenbach. Antimicrobial Activity of Cladonia verticillaris Lichen Preparations on Bacteria and Fungi of Medical Importance. Chinese Journal of Biology. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-455596
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-455596