Effect of Culture Conditions on the Production of an Extracellular Protease by Bacillus sp. Isolated from Soil Sample of Lavizan Jungle Park
Joint Authors
Akhavan Sepahy, Abbas
Jabalameli, Leila
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-07
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Soil samples of Tehran jungle parks were screened for proteolytic Bacilli.
Among eighteen protease producers one of the isolates obtained from Lavizan park, in north east of Tehran, was selected for further experimental studies.
This isolate was identified as Bacillus sp.
strain CR-179 based on partial sequencing of 16S rRNA.
Various nutritional and environmental parameters affected protease production by Bacillus sp.
strain CR-179.
Protease production by this Bacillus cultivated in liquid cultures reached a maximum at 24 h, with levels of 340.908 U/mL.
Starch and maltose were the best substrates for enzyme production while some pure sugars such as fructose, glucose, and sucrose could not influence production of protease.
Among various organic nitrogen sources corn steep liquor, which is commercial, was found as the best substrate followed by yeast extract, whey protein, and beef extract.
The optimal pH and optimal temperature of enzyme production were 8.0 and 45°C, respectively.
Studies on enzymatic characterization revealed that crude protease showed maximum activity at pH 9.0 and 60°C, which is indicating the enzyme to be thermoalkaline protease.
American Psychological Association (APA)
Akhavan Sepahy, Abbas& Jabalameli, Leila. 2011. Effect of Culture Conditions on the Production of an Extracellular Protease by Bacillus sp. Isolated from Soil Sample of Lavizan Jungle Park. Enzyme Research،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-455626
Modern Language Association (MLA)
Akhavan Sepahy, Abbas& Jabalameli, Leila. Effect of Culture Conditions on the Production of an Extracellular Protease by Bacillus sp. Isolated from Soil Sample of Lavizan Jungle Park. Enzyme Research No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-455626
American Medical Association (AMA)
Akhavan Sepahy, Abbas& Jabalameli, Leila. Effect of Culture Conditions on the Production of an Extracellular Protease by Bacillus sp. Isolated from Soil Sample of Lavizan Jungle Park. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-455626
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-455626