Efficacy of Continuously Administered PEDF-Derived Synthetic Peptides against Osteosarcoma Growth and Metastasis
Joint Authors
Dass, Crispin R.
Choong, Peter F. M.
Broadhead, Matthew L.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The potent antiangiogenic pigment epithelium-derived factor (PEDF) has shown promise against osteosarcoma, a tumour that originates in the bone and metastasises to the lungs.
Neurotrophic, antiangiogenic, antiproliferative, and antimetastatic properties of PEDF have been attributed to a number of functional epitopes on the PEDF glycoprotein.
StVOrth-2 (residues 78–102) and StVOrth-3 (residues 90–114) are two PEDF-derived peptides based on these functional epitopes.
StVOrth-2 has previously been shown to inhibit osteosarcoma cell proliferation, while StVOrth-3 increased osteosarcoma cell adhesion to collagen I in vitro.
In this paper, we have evaluated systemically and continuously delivered StVOrth-2 and StVOrth-3 using a clinically relevant murine model of osteosarcoma with spontaneous metastasis.
Treatment with StVOrth-2 or StVOrth-3 with microosmotic pumps was initiated after primary osteosarcoma was established in the tibia.
While treatment with StVOrth-2 and StVOrth-3 did not appear to affect local tumour invasion, tumour necrosis or apoptosis, StVOrth-2 predominantly restricted the growth of primary tumours, while StVOrth-3 restricted the burden of pulmonary metastatic disease.
No peptide caused gross toxicity in mouse tissues as assessed by measuring weight of animals, serum biochemistry, and gross tissue observation.
The differential effects exhibited by StVOrth-2 and StVOrth-3 in this orthotopic model of osteosarcoma may be related to the functional epitopes on the PEDF glycoprotein that they represent.
American Psychological Association (APA)
Broadhead, Matthew L.& Choong, Peter F. M.& Dass, Crispin R.. 2012. Efficacy of Continuously Administered PEDF-Derived Synthetic Peptides against Osteosarcoma Growth and Metastasis. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-455670
Modern Language Association (MLA)
Broadhead, Matthew L.…[et al.]. Efficacy of Continuously Administered PEDF-Derived Synthetic Peptides against Osteosarcoma Growth and Metastasis. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-455670
American Medical Association (AMA)
Broadhead, Matthew L.& Choong, Peter F. M.& Dass, Crispin R.. Efficacy of Continuously Administered PEDF-Derived Synthetic Peptides against Osteosarcoma Growth and Metastasis. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-455670
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-455670