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Identification of a Functional Type IA Topoisomerase, LdTopIIIβ, from Kinetoplastid Parasite Leishmania donovani
Joint Authors
Majumder, Hemanta K.
Sen, Nilkantha
Banerjee, Bijoylaxmi
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-02
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
DNA topoisomerases of kinetoplastids represent a family of DNA processing enzymes that essentially solve the topological problems not only in nuclear DNA but also in kinetoplast DNA.
We have, for the first time, identified a Leishmania donovani homologue of bacterial and eukaryotic IA type of topoisomerase III protein and termed as LdTopIIIβ.
Complementation study of wild-type and mutant LdTopIIIβ with slow-growing topoisomerase III mutant yeast S.
cerevisiae revealed the functional conservation of the leishmanial counterpart of topoisomerase IIIβ protein, the 327 tyrosine being the active site amino acid.
A C-terminal deletion construct of LdTopIIIβ could not suppress the slow-growth phenotype of mutant yeast, indicating the requirement of C-terminal region for the enzyme function in vivo.LdTopIIIβ localized inside the nucleus and kinetoplast of the parasite.
Taken together, our study indicates functional conservation and possible role of LdTopIIIβ in parasite DNA processing.
American Psychological Association (APA)
Banerjee, Bijoylaxmi& Sen, Nilkantha& Majumder, Hemanta K.. 2011. Identification of a Functional Type IA Topoisomerase, LdTopIIIβ, from Kinetoplastid Parasite Leishmania donovani. Enzyme Research،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-455696
Modern Language Association (MLA)
Banerjee, Bijoylaxmi…[et al.]. Identification of a Functional Type IA Topoisomerase, LdTopIIIβ, from Kinetoplastid Parasite Leishmania donovani. Enzyme Research No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-455696
American Medical Association (AMA)
Banerjee, Bijoylaxmi& Sen, Nilkantha& Majumder, Hemanta K.. Identification of a Functional Type IA Topoisomerase, LdTopIIIβ, from Kinetoplastid Parasite Leishmania donovani. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-455696
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-455696