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Discordant Correlation between Serological Assays Observed When Measuring Heterosubtypic Responses against Avian Influenza H5 and H7 Viruses in Unexposed Individuals
Joint Authors
Lapini, Giulia
Ferrara, Francesca
Molesti, Eleonora
Temperton, Nigel J.
Montomoli, Emanuele
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-11
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The human population is constantly exposed to multiple influenza A subtypes due to zoonotic spillover and rapid viral evolution driven by intrinsic error-prone replication and immunological pressure.
In this context, antibody responses directed against the HA protein are of importance since they have been shown to correlate with protective immunity.
Serological techniques, detecting these responses, play a critical role for influenza surveillance, vaccine development, and assessment.
As the recent human pandemics and avian influenza outbreaks have demonstrated, there is an urgent need to be better prepared to assess the contribution of the antibody response to protection against newly emerged viruses and to evaluate the extent of preexisting heterosubtypic immunity in populations.
In this study, 68 serum samples collected from the Italian population between 1992 and 2007 were found to be positive for antibodies against H5N1 as determined by single radial hemolysis (SRH), but most were negative when evaluated using haemagglutination inhibition (HI) and microneutralisation (MN) assays.
As a result of these discordant serological findings, the increased sensitivity of lentiviral pseudotypes was exploited in pseudotype-based neutralisation (pp-NT) assays and the results obtained provide further insight into the complex nature of humoral immunity against influenza A viruses.
American Psychological Association (APA)
Molesti, Eleonora& Ferrara, Francesca& Lapini, Giulia& Montomoli, Emanuele& Temperton, Nigel J.. 2014. Discordant Correlation between Serological Assays Observed When Measuring Heterosubtypic Responses against Avian Influenza H5 and H7 Viruses in Unexposed Individuals. BioMed Research International،Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-455781
Modern Language Association (MLA)
Molesti, Eleonora…[et al.]. Discordant Correlation between Serological Assays Observed When Measuring Heterosubtypic Responses against Avian Influenza H5 and H7 Viruses in Unexposed Individuals. BioMed Research International No. 2014 (2014), pp.1-12.
https://search.emarefa.net/detail/BIM-455781
American Medical Association (AMA)
Molesti, Eleonora& Ferrara, Francesca& Lapini, Giulia& Montomoli, Emanuele& Temperton, Nigel J.. Discordant Correlation between Serological Assays Observed When Measuring Heterosubtypic Responses against Avian Influenza H5 and H7 Viruses in Unexposed Individuals. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-12.
https://search.emarefa.net/detail/BIM-455781
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-455781