Ultrasensitive Nano-ELISA for Detecting Sulfadimethoxine in Chicken Tissue
Joint Authors
Xue, Feng
Peng, Chi-Fang
Duan, Xiao-Hui
Pan, Qiu-Li
Liu, Li-Qiang
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-29
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
It is challenging to obtain a highly sensitive enzyme-linked sorbent immunoassay (ELISA) method for the rapid screening of veterinary drug residue in animal tissues.
Here we present that a simple and sensitive detection for sulfadimethoxine (SDM) residue in animal tissues was achieved by utilizing a bioconjugate of gold nanoparticles and enzyme-labeled antibody as signal probe in enzyme-linked sorbent immunoassay (ELISA).
The developed nano-ELISA has increased the sensitivity of a traditional ELISA for SDM by 20-fold.
The sensitivity of this ELISA was 5 pg/mL in buffer, and the detection limit (LOD) of 0.2 µg/kg can be obtained after chicken liver was simply extracted by buffer.
This simple and sensitive method can be used to improve the sensitivity of ELISA methods for various small molecule contaminants.
American Psychological Association (APA)
Peng, Chi-Fang& Duan, Xiao-Hui& Pan, Qiu-Li& Liu, Li-Qiang& Xue, Feng. 2013. Ultrasensitive Nano-ELISA for Detecting Sulfadimethoxine in Chicken Tissue. Journal of Chemistry،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-455959
Modern Language Association (MLA)
Peng, Chi-Fang…[et al.]. Ultrasensitive Nano-ELISA for Detecting Sulfadimethoxine in Chicken Tissue. Journal of Chemistry No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-455959
American Medical Association (AMA)
Peng, Chi-Fang& Duan, Xiao-Hui& Pan, Qiu-Li& Liu, Li-Qiang& Xue, Feng. Ultrasensitive Nano-ELISA for Detecting Sulfadimethoxine in Chicken Tissue. Journal of Chemistry. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-455959
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-455959