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Fluorescence-Based Rapid Detection of Microbiological Contaminants in Water Samples
Joint Authors
Chollet, Renaud
Olivieri, Frédéric
Welterlin, Esther
Beguin, Vincent
Kukuczka, Monika
Barrier, Sophie
Ribault, Sébastien
Meder, Hervé
Baumstummler, Anne
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
Microbiological contamination of process waters is a current issue for pharmaceutical industries.
Traditional methods require several days to obtain results; therefore, rapid microbiological methods are widely requested to shorten time-to-result.
Milliflex Quantum was developed for the rapid detection and enumeration of microorganisms in filterable samples.
It combines membrane filtration to universal fluorescent staining of viable microorganisms.
This new alternative method was validated using European and United States Pharmacopeia definitions, with sterile water and/or sterile water artificially contaminated with microorganisms.
The Milliflex Quantum method was demonstrated to be reliable, robust, specific, accurate, and linear over the whole range of assays following these guidelines.
The Milliflex Quantum system was challenged to detect natural contaminants in different types of pharmaceutical purified process waters.
Milliflex Quantum was demonstrated to detect accurately contaminants 3- to 7-fold faster than traditional membrane filtration method.
The staining procedure is nondestructive allowing downstream identification following a positive result.
The Milliflex Quantum offers a fast, sensitive, and robust alternative to the compendial membrane filtration method.
American Psychological Association (APA)
Meder, Hervé& Baumstummler, Anne& Chollet, Renaud& Barrier, Sophie& Kukuczka, Monika& Olivieri, Frédéric…[et al.]. 2012. Fluorescence-Based Rapid Detection of Microbiological Contaminants in Water Samples. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-456014
Modern Language Association (MLA)
Meder, Hervé…[et al.]. Fluorescence-Based Rapid Detection of Microbiological Contaminants in Water Samples. The Scientific World Journal No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-456014
American Medical Association (AMA)
Meder, Hervé& Baumstummler, Anne& Chollet, Renaud& Barrier, Sophie& Kukuczka, Monika& Olivieri, Frédéric…[et al.]. Fluorescence-Based Rapid Detection of Microbiological Contaminants in Water Samples. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-456014
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-456014