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Characterization of PEBBLEs as a Tool for Real-Time Measurement of Dictyostelium discoideum Endosomal pH
Joint Authors
Moding, Everett
Hellyer, Jessica
Lostroh, Phoebe
Brasuel, Murphy
Rank, Kevin
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-08-17
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
The measurement of intracellular ion concentration change is important for understanding the cellular mechanisms for communication.
Recently developed nanosensors, (Photonic Explorers for Biomedical use with Biologically Localized Embedding) PEBBLEs, have a number of advantages for measuring ions in cells over established methods using microelectrodes, unbound fluorescent dyes, or NMR.
PEBBLE sensors have been shown to work in principle for measuring dynamic ion changes, but few in vivo applications have been demonstrated.
We modified the protocol for the fabrication of pH sensing PEBBLEs and developed a protocol for the utilization of these sensors for the monitoring of dynamic pH changes in the endosomes of slime mold Dictyostelium discoideum (D.
discoideum).
Oregon Green 514-CdSe Quantum Dot PEBBLEs were used to measure real-time pH inside D.
discoideum endosomes during cAMP stimulation.
Endosomal pH was shown to decrease during cAMP signaling, demonstrating a movement of protons into the endosomes of D.
discoideum amoebae.
American Psychological Association (APA)
Moding, Everett& Hellyer, Jessica& Rank, Kevin& Lostroh, Phoebe& Brasuel, Murphy. 2009. Characterization of PEBBLEs as a Tool for Real-Time Measurement of Dictyostelium discoideum Endosomal pH. Journal of Sensors،Vol. 2009, no. 2009, pp.1-4.
https://search.emarefa.net/detail/BIM-456037
Modern Language Association (MLA)
Moding, Everett…[et al.]. Characterization of PEBBLEs as a Tool for Real-Time Measurement of Dictyostelium discoideum Endosomal pH. Journal of Sensors No. 2009 (2009), pp.1-4.
https://search.emarefa.net/detail/BIM-456037
American Medical Association (AMA)
Moding, Everett& Hellyer, Jessica& Rank, Kevin& Lostroh, Phoebe& Brasuel, Murphy. Characterization of PEBBLEs as a Tool for Real-Time Measurement of Dictyostelium discoideum Endosomal pH. Journal of Sensors. 2009. Vol. 2009, no. 2009, pp.1-4.
https://search.emarefa.net/detail/BIM-456037
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-456037