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Fibroblast Growth Factor-2 Enhances Expansion of Human Bone Marrow-Derived Mesenchymal Stromal Cells without Diminishing Their Immunosuppressive Potential
Joint Authors
Solchaga, Luis A.
Welter, Jean F.
Auletta, Jeffery J.
Zale, Elizabeth A.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-03-03
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Allogeneic hematopoietic stem cell transplantation is the main curative therapy for many hematologic malignancies.
Its potential relies on graft-versus-tumor effects which associate with graft-versus-host disease.
Mesenchymal stromal cells (MSCs) possess immunomodulatory properties that make them attractive therapeutic alternatives.
We evaluated the in vitro immunosuppressive activity of medium conditioned by human MSCs from 5 donors expanded 13 passages with or without FGF-2.
FGF-2 supplementation increased expansion 3,500- and 240,000-fold by passages 7 and 13, respectively.
There were no differences in immunosuppressive activity between media conditioned by passage-matched cells expanded under different conditions, but media conditioned by FGF-treated MSCs were superior to population doubling-matched controls.
The immunosuppressive activity was maintained in three of the preparations but decreased with expansion in two.
The proliferation induced by FGF-2 did not result in loss of immunosuppressive activity.
However, because the immunosuppressive activity was not consistently preserved, caution must be exercised to ensure that the activity of the cells is sufficient after extensive expansion.
American Psychological Association (APA)
Auletta, Jeffery J.& Zale, Elizabeth A.& Welter, Jean F.& Solchaga, Luis A.. 2011. Fibroblast Growth Factor-2 Enhances Expansion of Human Bone Marrow-Derived Mesenchymal Stromal Cells without Diminishing Their Immunosuppressive Potential. Stem Cells International،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-456038
Modern Language Association (MLA)
Auletta, Jeffery J.…[et al.]. Fibroblast Growth Factor-2 Enhances Expansion of Human Bone Marrow-Derived Mesenchymal Stromal Cells without Diminishing Their Immunosuppressive Potential. Stem Cells International No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-456038
American Medical Association (AMA)
Auletta, Jeffery J.& Zale, Elizabeth A.& Welter, Jean F.& Solchaga, Luis A.. Fibroblast Growth Factor-2 Enhances Expansion of Human Bone Marrow-Derived Mesenchymal Stromal Cells without Diminishing Their Immunosuppressive Potential. Stem Cells International. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-456038
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-456038