Development of Transgenic Papaya through Agrobacterium-Mediated Transformation
Joint Authors
Azad, Md. Abul Kalam
Amin, Latifah
Rabbani, Md. Golam
Sidik, Nik Marzuki
Source
International Journal of Genomics
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-28
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Transgenic papaya plants were regenerated from hypocotyls and immature zygotic embryo after cocultivation with Agrobacterium tumefaciens LBA-4404 carrying a binary plasmid vector system containing neomycin phosphotransferase (nptII) gene as the selectable marker and β-glucuronidase (GUS) as the reporter gene.
The explants were co-cultivated with Agrobacterium tumefaciens on regeneration medium containing 500 mg/L carbenicillin + 200 mg/L cefotaxime for one week.
The cocultivated explants were transferred into the final selection medium containing 500 mg/L carbenicillin + 200 mg/L cefotaxime + 50 mg/L kanamycin for callus induction as well as plant regeneration.
The callus derived from the hypocotyls of Carica papaya cv.
Shahi showed the highest positive GUS activities compared to Carica papaya cv.
Ranchi.
The transformed callus grew vigorously and formed embryos followed by transgenic plantlets successfully.
The result of this study showed that the hypocotyls of C.
papaya cv.
Shahi and C.
papaya cv.
Ranchi are better explants for genetic transformation compared to immature embryos.
The transformed C.
papaya cv.
Shahi also showed the maximum number of plant regeneration compared to that of C.
papaya cv.
Ranchi.
American Psychological Association (APA)
Azad, Md. Abul Kalam& Rabbani, Md. Golam& Amin, Latifah& Sidik, Nik Marzuki. 2013. Development of Transgenic Papaya through Agrobacterium-Mediated Transformation. International Journal of Genomics،Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-456072
Modern Language Association (MLA)
Azad, Md. Abul Kalam…[et al.]. Development of Transgenic Papaya through Agrobacterium-Mediated Transformation. International Journal of Genomics No. 2013 (2013), pp.1-5.
https://search.emarefa.net/detail/BIM-456072
American Medical Association (AMA)
Azad, Md. Abul Kalam& Rabbani, Md. Golam& Amin, Latifah& Sidik, Nik Marzuki. Development of Transgenic Papaya through Agrobacterium-Mediated Transformation. International Journal of Genomics. 2013. Vol. 2013, no. 2013, pp.1-5.
https://search.emarefa.net/detail/BIM-456072
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-456072