Caenorhabditis elegans F09E10.3 Encodes a Putative 3-Oxoacyl-Thioester Reductase of Mitochondrial Type 2 Fatty Acid Synthase FASII that Is Functional in Yeast
Author
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-09-07
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Caenorhabditis elegans F09E10.3 (dhs-25) was identified as encoding a 3-oxoacyl-thioester reductase, potentially of the mitochondrial type 2 fatty acid synthase (FASII) system.
Mitochondrial FASII is a relatively recent discovery in metazoans, and the relevance of this process to animal physiology has not been elucidated.
A good animal model to study the role of FASII is the nematode C.
elegans.
However, the components of nematode mitochondrial FASII have hitherto evaded positive identification.
The nematode F09E10.3 protein was ectopically expressed without an additional mitochondrial targeting sequence in Saccharomyces cerevisiae mutant cells lacking the homologous mitochondrial FASII enzyme 3-oxoacyl-ACP reductase Oar1p.
These yeast oar1Δ mutants are unable to respire, grow on nonfermentable carbon sources, or synthesize sufficient levels of lipoic acid.
Mutant yeast cells producing a full-length mitochondrial F09E10.3 protein contained NAD+-dependent 3-oxoacyl-thioester reductase activity and resembled the corresponding mutant overexpressing native Oar1p for the above-mentioned phenotype characteristics.
This is the first identification of a metazoan 3-oxoacyl-thioester reductase (see Note Added in Proof).
American Psychological Association (APA)
Gurvitz, Aner. 2009. Caenorhabditis elegans F09E10.3 Encodes a Putative 3-Oxoacyl-Thioester Reductase of Mitochondrial Type 2 Fatty Acid Synthase FASII that Is Functional in Yeast. Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-6.
https://search.emarefa.net/detail/BIM-456100
Modern Language Association (MLA)
Gurvitz, Aner. Caenorhabditis elegans F09E10.3 Encodes a Putative 3-Oxoacyl-Thioester Reductase of Mitochondrial Type 2 Fatty Acid Synthase FASII that Is Functional in Yeast. Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-6.
https://search.emarefa.net/detail/BIM-456100
American Medical Association (AMA)
Gurvitz, Aner. Caenorhabditis elegans F09E10.3 Encodes a Putative 3-Oxoacyl-Thioester Reductase of Mitochondrial Type 2 Fatty Acid Synthase FASII that Is Functional in Yeast. Journal of Biomedicine and Biotechnology. 2009. Vol. 2009, no. 2009, pp.1-6.
https://search.emarefa.net/detail/BIM-456100
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-456100