Ischemic Preconditioning of Rat Livers from Non-Heart-Beating Donors Decreases Parenchymal Cell Killing and Increases Graft Survival after Transplantation
Joint Authors
Peng, Xing-Xi
Currin, Robert T.
Lemasters, John J.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-19
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A critical shortage of donors exists for liver transplantation, which non-heart-beating cadaver donors could help ease.
This study evaluated ischemic preconditioning to improve graft viability after non-heart-beating liver donation in rats.
Ischemic preconditioning was performed by clamping the portal vein and hepatic artery for 10 min followed by unclamping for 5 min.
Subsequently, the aorta was cross-clamped for up to 120 min.
After 2 h of storage, livers were either transplanted or perfused with warm buffer containing trypan blue.
Aortic clamping for 60 and 120 min prior to liver harvest markedly decreased 30-day graft survival from 100% without aortic clamping to 50% and 0%, respectively, which ischemic preconditioning restored to 100 and 50%.
After 60 min of aortic clamping, loss of viability of parenchymal and nonparenchymal cells was 22.6 and 5.6%, respectively, which preconditioning decreased to 3.0 and 1.5%.
Cold storage after aortic clamping further increased parenchymal and non-parenchymal cell killing to 40.4 and 10.1%, respectively, which ischemic preconditioning decreased to 12.4 and 1.8%.
In conclusion, ischemic preconditioning markedly decreased cell killing after subsequent sustained warm ischemia.
Most importantly, ischemic preconditioning restored 100% graft survival of livers harvested from non-heart-beating donors after 60 min of aortic clamping.
American Psychological Association (APA)
Currin, Robert T.& Peng, Xing-Xi& Lemasters, John J.. 2012. Ischemic Preconditioning of Rat Livers from Non-Heart-Beating Donors Decreases Parenchymal Cell Killing and Increases Graft Survival after Transplantation. HPB Surgery،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-456157
Modern Language Association (MLA)
Currin, Robert T.…[et al.]. Ischemic Preconditioning of Rat Livers from Non-Heart-Beating Donors Decreases Parenchymal Cell Killing and Increases Graft Survival after Transplantation. HPB Surgery No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-456157
American Medical Association (AMA)
Currin, Robert T.& Peng, Xing-Xi& Lemasters, John J.. Ischemic Preconditioning of Rat Livers from Non-Heart-Beating Donors Decreases Parenchymal Cell Killing and Increases Graft Survival after Transplantation. HPB Surgery. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-456157
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-456157