Octanoylated Ghrelin Inhibits the Activation of the Palmitic Acid-Induced TLR4NF-κB Signaling Pathway in THP-1 Macrophages
Joint Authors
He, L. N.
Li, X. Y.
Li, Z.
Yan, K.
Xiao, Y.
Liu, S. P.
Zhou, Z. G.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
To investigate the effect of acylated ghrelin on the activation of TLR4/NF-κB signaling pathway induced by palmitic acid in human monocyte-derived (THP-1) macrophages, THP-1 macrophages were cultured for 12 h by palmitic acid with various concentrations.
The THP-1 macrophages was pretreated by acylated ghrelin at different doses for 4 h before cultivated by palmitic acid (200 μmol/L) for 12 h.
We observed the level of TLR4, NF-κB p65 phosphorylation in THP-1 macrophages and TNF-α, IL-1β in culture supernatant.
TLR4 mRNA was measured by real-time PCR.
TLR4 protein and NF-κB p65 phosphorylation was measured by western blotting.
The expression of TNF-α and IL-1β was detected by ELISA.
Compared to the THP-1 macrophages without palmitic acid, the level of TLR4 mRNA protein and NF-κB p65 phosphorylation and the expression of TNF-α and IL-1β increased after treatment by palmitic acid in a dose-dependent fashion (P<0.05).
Compared to the THP-1 macrophages with palmitic acid (200 μmol/L), the level of the pervious substances decreased after preadministration by acylated ghrelin in a dose-dependent fashion.
So, we make a conclusion that acylated ghrelin can regulate the activation of TLR4/NF-κB signaling pathway and inhibit the release of inflammatory cytokines in THP-1 macrophages which are stimulated by palmitic acid in a dose-dependent fashion.
American Psychological Association (APA)
Liu, S. P.& Li, X. Y.& Li, Z.& He, L. N.& Xiao, Y.& Yan, K.…[et al.]. 2012. Octanoylated Ghrelin Inhibits the Activation of the Palmitic Acid-Induced TLR4NF-κB Signaling Pathway in THP-1 Macrophages. ISRN Endocrinology،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-456264
Modern Language Association (MLA)
Liu, S. P.…[et al.]. Octanoylated Ghrelin Inhibits the Activation of the Palmitic Acid-Induced TLR4NF-κB Signaling Pathway in THP-1 Macrophages. ISRN Endocrinology No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-456264
American Medical Association (AMA)
Liu, S. P.& Li, X. Y.& Li, Z.& He, L. N.& Xiao, Y.& Yan, K.…[et al.]. Octanoylated Ghrelin Inhibits the Activation of the Palmitic Acid-Induced TLR4NF-κB Signaling Pathway in THP-1 Macrophages. ISRN Endocrinology. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-456264
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-456264