Recovery of Bacillus licheniformis Alkaline Protease from Supernatant of Fermented Wastewater Sludge Using Ultrafiltration and Its Characterization
Joint Authors
John, Rojan P.
Surampalli, R. Y.
Yan, S.
Bezawada, Jyothi
Tyagi, R. D.
Source
Biotechnology Research International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-18
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Information Technology and Computer Science
Abstract EN
Investigation on recovery of alkaline protease from B.
licheniformis ATCC 21424 fermented wastewater sludge was carried out by centrifugation and ultrafiltration.
Optimization of ultrafiltration parameters (transmembrane pressure (TMP) and feed flux) was carried out with 10 kDa membrane.
TMP of 90 kPa and feed flux of 714 L/h/m2 gave highest recovery (83%) of the enzyme from the centrifuged supernatant.
The recovered enzyme had given maximum activity at temperature of 60°C and at pH 10.
It was stable between pH 8 to 10 and retained 97% activity at 60°C after 180 min of incubation.
Enzyme activity was significantly augmented by metal ions like Ca2+ and Mn2+.
Protease inhibitors like phenylmethyl sulphonyl fluoride (PMSF) and diisopropyl fluorophosphates (DFPs) completely inhibited the enzyme activity.
The partially purified protease showed excellent stability and compatibility with various commercial detergents.
The detergent (Sunlight) removed the blood stains effectively along with the enzyme as additive.
American Psychological Association (APA)
Bezawada, Jyothi& Yan, S.& John, Rojan P.& Tyagi, R. D.& Surampalli, R. Y.. 2011. Recovery of Bacillus licheniformis Alkaline Protease from Supernatant of Fermented Wastewater Sludge Using Ultrafiltration and Its Characterization. Biotechnology Research International،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-456360
Modern Language Association (MLA)
Bezawada, Jyothi…[et al.]. Recovery of Bacillus licheniformis Alkaline Protease from Supernatant of Fermented Wastewater Sludge Using Ultrafiltration and Its Characterization. Biotechnology Research International No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-456360
American Medical Association (AMA)
Bezawada, Jyothi& Yan, S.& John, Rojan P.& Tyagi, R. D.& Surampalli, R. Y.. Recovery of Bacillus licheniformis Alkaline Protease from Supernatant of Fermented Wastewater Sludge Using Ultrafiltration and Its Characterization. Biotechnology Research International. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-456360
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-456360