Occurrence of Bordetella Infection in Pigs in Northern India
Joint Authors
Singh, Bhoj R.
Singh, Vidya
Kumar, Sandeep
Bhardwaj, Monika
Source
International Journal of Microbiology
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-12
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
Bordetella bronchiseptica infection causing atrophic rhinitis in pigs is reported from almost all countries.
In the present study, occurrence of Bordetella infection in apparently healthy pigs was determined in 392 pigs sampled to collect 358 serum samples and 316 nasal swabs from Northern India by conventional bacterioscopy, detection of antigen with multiplex polymerase chain reaction (mPCR), and detection of antibodies with microagglutination test (MAT) and enzyme linked immune-sorbent assay (ELISA).
Bordetella bronchiseptica could be isolated from six (1.92%) nasal swabs.
Although isolates varied significantly in their antimicrobial sensitivity, they had similar plasmid profile.
The genus specific and species specific amplicons were detected from 8.2% and 4.4% nasal swabs using mPCR with alc gene (genus specific) and fla gene and fim2 gene (species specific) primers, respectively.
Observations revealed that there may be other bordetellae infecting pigs because about 50% of the samples positive using mPCR for genus specific amplicons failed to confirm presence of B.
bronchiseptica.
Of the pig sera tested with MAT and ELISA for Bordetella antibodies, 67.6% and 86.3% samples, respectively, were positive.
For antigen detection mPCR was more sensitive than conventional bacterioscopy while for detection of antibodies neither of the two tests (MAT and ELISA) had specificity in relation to antigen detection.
Study indicated high prevalence of infection in swine herds in Northern India.
American Psychological Association (APA)
Kumar, Sandeep& Singh, Bhoj R.& Bhardwaj, Monika& Singh, Vidya. 2014. Occurrence of Bordetella Infection in Pigs in Northern India. International Journal of Microbiology،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-456364
Modern Language Association (MLA)
Kumar, Sandeep…[et al.]. Occurrence of Bordetella Infection in Pigs in Northern India. International Journal of Microbiology No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-456364
American Medical Association (AMA)
Kumar, Sandeep& Singh, Bhoj R.& Bhardwaj, Monika& Singh, Vidya. Occurrence of Bordetella Infection in Pigs in Northern India. International Journal of Microbiology. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-456364
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-456364