![](/images/graphics-bg.png)
Chemical Synthesis, Characterisation, and Biocompatibility of Nanometre Scale Porous Anodic Aluminium Oxide Membranes for Use as a Cell Culture Substrate for the Vero Cell Line : A Preliminary Study
Joint Authors
Fawcett, Derek
Le, Xuan Thi
Becker, Thomas
O'Dea, Mark
Poinern, Gérrard Eddy Jai
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-21
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
In this preliminary study we investigate for the first time the biomedical potential of using porous anodic aluminium oxide (AAO) membranes as a cell substrate for culturing the Cercopithecus aethiops (African green monkey) Kidney (Vero) epithelial cell line.
One advantage of using the inorganic AAO membrane is the presence of nanometre scale pore channels that allow the exchange of molecules and nutrients across the membrane.
The size of the pore channels can be preselected by adjusting the controlling parameters of a temperature controlled two-step anodization process.
The cellular interaction and response of the Vero cell line with an in-house synthesised AAO membrane, a commercially available membrane, and a glass control were assessed by investigating cell adhesion, morphology, and proliferation over a 72 h period.
The number of viable cells proliferating over the respective membrane surfaces revealed that the locally produced in-house AAO membrane had cells numbers similar to the glass control.
The study revealed evidence of focal adhesion sites over the surface of the nanoporous membranes and the penetration of cellular extensions into the pore structure as well.
The outcome of the study has revealed that nanometre scale porous AAO membranes have the potential to become practical cell culture scaffold substrates with the capability to enhance adhesion and proliferation of Vero cells.
American Psychological Association (APA)
Poinern, Gérrard Eddy Jai& Le, Xuan Thi& O'Dea, Mark& Becker, Thomas& Fawcett, Derek. 2014. Chemical Synthesis, Characterisation, and Biocompatibility of Nanometre Scale Porous Anodic Aluminium Oxide Membranes for Use as a Cell Culture Substrate for the Vero Cell Line : A Preliminary Study. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-456389
Modern Language Association (MLA)
Poinern, Gérrard Eddy Jai…[et al.]. Chemical Synthesis, Characterisation, and Biocompatibility of Nanometre Scale Porous Anodic Aluminium Oxide Membranes for Use as a Cell Culture Substrate for the Vero Cell Line : A Preliminary Study. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-456389
American Medical Association (AMA)
Poinern, Gérrard Eddy Jai& Le, Xuan Thi& O'Dea, Mark& Becker, Thomas& Fawcett, Derek. Chemical Synthesis, Characterisation, and Biocompatibility of Nanometre Scale Porous Anodic Aluminium Oxide Membranes for Use as a Cell Culture Substrate for the Vero Cell Line : A Preliminary Study. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-456389
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-456389