Elements of the B Cell Signalosome Are Differentially Affected by Mercury Intoxication
Joint Authors
Gill, Randall F.
Rosenspire, Allen J.
McCabe, Michael J.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-05-04
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
It has been suggested that environmental exposures to mercury contribute to autoimmune disease.
Disruption of BCR signaling is associated with failure of central tolerance and autoimmunity, and we have previously shown that low levels of Hg2+ interfere with BCR signaling.
In this report we have employed multiparametric phosphoflow cytometry, as well as a novel generalization of the Overton algorithm from one- to two-dimensional unimodal distributions to simultaneously monitor the effect of low level Hg2+ intoxication on activation of ERK and several upstream elements of the BCR signaling pathway in WEHI-231 B cells.
We have found that, after exposure to low levels of Hg2+, only about a third of the cells are sensitive to the metal.
For those cells which are sensitive, we confirm our earlier work that activation of ERK is attenuated but now report that Hg2+ has little upstream effect on the Btk tyrosine kinase.
On the other hand, we find that signaling upstream through the Syk tyrosine kinase is actually augmented, as is upstream activation of the B cell signalosome scaffolding protein BLNK.
American Psychological Association (APA)
Gill, Randall F.& McCabe, Michael J.& Rosenspire, Allen J.. 2014. Elements of the B Cell Signalosome Are Differentially Affected by Mercury Intoxication. Autoimmune Diseases،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-456449
Modern Language Association (MLA)
Gill, Randall F.…[et al.]. Elements of the B Cell Signalosome Are Differentially Affected by Mercury Intoxication. Autoimmune Diseases No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-456449
American Medical Association (AMA)
Gill, Randall F.& McCabe, Michael J.& Rosenspire, Allen J.. Elements of the B Cell Signalosome Are Differentially Affected by Mercury Intoxication. Autoimmune Diseases. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-456449
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-456449