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Mechanism of Sperm Immobilization by Escherichia coli
Joint Authors
Kaur, Kiranjeet
Singh, Shrawan Kumar
Sandhu, Ravneet
Sarwal, Abha
Mavuduru, Ravimohan S.
Kaur, Siftjit
Prabha, Vijay
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-03-30
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Aim.
To explore the influence of Escherichia coli on the motility of human spermatozoa and its possible mechanism.
Methods.
Highly motile preparations of spermatozoa from normozoospermic patients were coincubated with Escherichia coli for 4 hours.
At 1, 2 and 4 hours of incubation, sperm motility was determined.
The factor responsible for sperm immobilization without agglutination was isolated and purified from filtrates.
Results.
This report confirms the immobilization of spermatozoa by E.
coli and demonstrates sperm immobilization factor (SIF) excreted by E.
coli.
Further this factor was purified by ammonium sulfate precipitation, gel permeation chromatography, and ion-exchange chromatography.
Purified SIF (56 kDa) caused instant immobilization without agglutination of human spermatozoa at 800 μg/mL and death at 2.1 mg/mL.
Spermatozoa incubated with SIF revealed multiple and profound alterations involving all superficial structures of spermatozoa as observed by scanning electron microscopy.
Conclusion.
In conclusion, these results have shown immobilization of spermatozoa by E.
coli and demonstrate a factor (SIF) produced and secreted by E.
coli which causes variable structural damage as probable morphological correlates of immobilization.
American Psychological Association (APA)
Prabha, Vijay& Sandhu, Ravneet& Kaur, Siftjit& Kaur, Kiranjeet& Sarwal, Abha& Mavuduru, Ravimohan S.…[et al.]. 2010. Mechanism of Sperm Immobilization by Escherichia coli. Advances in Urology،Vol. 2010, no. 2010, pp.1-6.
https://search.emarefa.net/detail/BIM-456525
Modern Language Association (MLA)
Prabha, Vijay…[et al.]. Mechanism of Sperm Immobilization by Escherichia coli. Advances in Urology No. 2010 (2010), pp.1-6.
https://search.emarefa.net/detail/BIM-456525
American Medical Association (AMA)
Prabha, Vijay& Sandhu, Ravneet& Kaur, Siftjit& Kaur, Kiranjeet& Sarwal, Abha& Mavuduru, Ravimohan S.…[et al.]. Mechanism of Sperm Immobilization by Escherichia coli. Advances in Urology. 2010. Vol. 2010, no. 2010, pp.1-6.
https://search.emarefa.net/detail/BIM-456525
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-456525