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Tyrosinase Small Interfering RNA Effectively Suppresses Tyrosinase Gene Expression In Vitro and In Vivo
Joint Authors
Jing, Zhuang
Xiang, Zhu
Ting, Luo
Shao-Chun, Lin
Wei-Hua, Li
Xi-Gu, Chen
Bing, Huang
Qian, Liu
Xiu-Hua, Jia
Xiao-Ping, Xu
Source
Molecular Biology International
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-11-04
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Tyrosinase is a bifunctional enzyme which oxidizes the initial step of melanin biosynthesis, that is, conversion of tyrosine to dopa and subsequently dopa to dopaquinone.
It is a glycosylated protein and a major regulator of melanogenesis.
To date, many approaches have been tried to regulate tyrosinase activity and melanin content.
To that end, we screened small interfering RNA sequences for sequence-inhibited tyrosinase expression in B16 cells and in C57BL/6 mice.
We analyzed tyrosinase mRNA levels by quantitative real-time PCR and determined tyrosinase activity and melanin content at 24, 48, and 72 hours after transfection.
Results showed that siNM_011661_001 was the most efficient small interfering RNA sequence in suppressing tyrosinase mRNA expression, and cells transfected with this sequence showed lower tyrosinase activity.
Moreover, intravitreous injection of siNM_011661_001 in C57BL/6 mice induced an efficient and stable gene-specific inhibition of expression at the posttranscriptional level.
American Psychological Association (APA)
Xiu-Hua, Jia& Shao-Chun, Lin& Bing, Huang& Xiang, Zhu& Jing, Zhuang& Wei-Hua, Li…[et al.]. 2010. Tyrosinase Small Interfering RNA Effectively Suppresses Tyrosinase Gene Expression In Vitro and In Vivo. Molecular Biology International،Vol. 2010, no. 2010, pp.1-6.
https://search.emarefa.net/detail/BIM-456549
Modern Language Association (MLA)
Xiu-Hua, Jia…[et al.]. Tyrosinase Small Interfering RNA Effectively Suppresses Tyrosinase Gene Expression In Vitro and In Vivo. Molecular Biology International No. 2010 (2010), pp.1-6.
https://search.emarefa.net/detail/BIM-456549
American Medical Association (AMA)
Xiu-Hua, Jia& Shao-Chun, Lin& Bing, Huang& Xiang, Zhu& Jing, Zhuang& Wei-Hua, Li…[et al.]. Tyrosinase Small Interfering RNA Effectively Suppresses Tyrosinase Gene Expression In Vitro and In Vivo. Molecular Biology International. 2010. Vol. 2010, no. 2010, pp.1-6.
https://search.emarefa.net/detail/BIM-456549
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-456549