RP-HPLC Method for Determination of Several NSAIDs and Their Combination Drugs

Abstract EN

An RP-HPLC method for simultaneous determination of 9 NSAIDs (paracetamol, salicylic acid, ibuprofen, naproxen, aceclofenac, diclofenac, ketorolac, etoricoxib, and aspirin) and their commonly prescribed combination drugs (thiocolchicoside, moxifloxacin, clopidogrel, chlorpheniramine maleate, dextromethorphan, and domperidone) was established.

The separation was performed on Kromasil C18 (250 × 4.6 mm, 5 μm) at 35°C using 15 mM phosphate buffer pH 3.25 and acetonitrile with gradient elution at a flow rate of 1.1 mL/min.

The detection was performed by a diode array detector (DAD) at 230 nm with total run time of 30 min.

Calibration curves were linear with correlation coefficients of determination r2>0.999.

Limit of detection (LOD) and Limit of quantification (LOQ) ranged from 0.04 to 0.97 μg/mL and from 0.64 to 3.24 μg/mL, respectively.

As an application tool of quality by design, full factorial experimental design was used for the testing of robustness of the method.

The prediction profiler correlating various parameters and responses was established from the results of design of experiments (DOE).