Sample Preparation Techniques for the Untargeted LC-MS-Based Discovery of Peptides in Complex Biological Matrices
Joint Authors
Pinkse, Martijn W. H.
Verhaert, Peter D. E. M.
Van Dongen, William
Finoulst, Inez
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-12
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Although big progress has been made in sample pretreatment over the last years, there are still considerable limitations when it comes to overcoming complexity and dynamic range problems associated with peptide analyses from biological matrices.
Being the little brother of proteomics, peptidomics is a relatively new field of research aiming at the direct analysis of the small proteins, called peptides, many of which are not amenable for typical trypsin-based analytics.
In this paper, we present an overview of different techniques and methods currently used for reducing a sample's complexity and for concentrating low abundant compounds to enable successful peptidome analysis.
We focus on techniques which can be employed prior to liquid chromatography coupled to mass spectrometry for peptide detection and identification and indicate their advantages as well as their shortcomings when it comes to the untargeted analysis of native peptides from complex biological matrices.
American Psychological Association (APA)
Finoulst, Inez& Pinkse, Martijn W. H.& Van Dongen, William& Verhaert, Peter D. E. M.. 2011. Sample Preparation Techniques for the Untargeted LC-MS-Based Discovery of Peptides in Complex Biological Matrices. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-14.
https://search.emarefa.net/detail/BIM-456882
Modern Language Association (MLA)
Finoulst, Inez…[et al.]. Sample Preparation Techniques for the Untargeted LC-MS-Based Discovery of Peptides in Complex Biological Matrices. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-14.
https://search.emarefa.net/detail/BIM-456882
American Medical Association (AMA)
Finoulst, Inez& Pinkse, Martijn W. H.& Van Dongen, William& Verhaert, Peter D. E. M.. Sample Preparation Techniques for the Untargeted LC-MS-Based Discovery of Peptides in Complex Biological Matrices. Journal of Biomedicine and Biotechnology. 2011. Vol. 2011, no. 2011, pp.1-14.
https://search.emarefa.net/detail/BIM-456882
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-456882