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Proteomic Profiling of Ex Vivo Expanded CD34-Positive Haematopoetic Cells Derived from Umbilical Cord Blood
Joint Authors
Falkenberg, Heiner
Kogler, Gesine
Radke, Teja Falk
Stühler, Kai
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-26
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Ex vivo expansion of haematopoetic cells by application of specific cytokines is one approach to overcome boundaries in cord blood transplantation due to limited numbers of haematopoetic stem cells.
While many protocols describe an effective increase of total cell numbers and the amount of CD34-positive cells, it still remains unclear if and how the procedure actually affects the cells’ properties.
In the presented publications, CD34-positive cells were isolated from cord blood and expanded for up to 7 days in media supplemented with stem cell factor (SCF), thrombopoietin (THPO), interleukin 6 (IL-6), and fms-related tyrosine kinase 3 ligand (FLT3lg).
At days 3 and 7, expanded cells were harvested and analyzed by flow cytometry and quantitative proteomics.
2970 proteins were identified, whereof proteomic analysis showed 440 proteins significantly changed in abundance during ex vivo expansion.
Despite the fact that haematopoetic cells still expressed CD34 on the surface after 3 days, major changes in regard to the protein profile were observed, while further expansion showed less effect on the proteome level.
Enrichment analysis of biological processes clearly showed a proteomic change toward a protein biosynthesis phenotype already within the first three days of expression.
American Psychological Association (APA)
Falkenberg, Heiner& Radke, Teja Falk& Kogler, Gesine& Stühler, Kai. 2013. Proteomic Profiling of Ex Vivo Expanded CD34-Positive Haematopoetic Cells Derived from Umbilical Cord Blood. Stem Cells International،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-456925
Modern Language Association (MLA)
Falkenberg, Heiner…[et al.]. Proteomic Profiling of Ex Vivo Expanded CD34-Positive Haematopoetic Cells Derived from Umbilical Cord Blood. Stem Cells International No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-456925
American Medical Association (AMA)
Falkenberg, Heiner& Radke, Teja Falk& Kogler, Gesine& Stühler, Kai. Proteomic Profiling of Ex Vivo Expanded CD34-Positive Haematopoetic Cells Derived from Umbilical Cord Blood. Stem Cells International. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-456925
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-456925