IRF5 Is a Specific Marker of Inflammatory Macrophages In Vivo
Joint Authors
Udalova, Irina A.
Weiss, Miriam
Blazek, Katrina
Perocheau, Dany P.
Byrne, Adam J.
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-23
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Macrophages are an integral part of the innate immune system and key players in pathogen clearance and tissue remodelling.
Both functions are accomplished by a pivotal network of different macrophage subtypes, including proinflammatory M1 and anti-inflammatory M2 macrophages.
Previously, our laboratory identified the transcription factor interferon regulatory factor 5 (IRF5) as the master regulator of the M1 macrophage polarisation.
IRF5 was found to be highly expressed in human M1 compared to M2 macrophages.
Furthermore, IRF5 dictates the expression of proinflammatory genes such as IL12b and IL23a whilst repressing anti-inflammatory genes like IL10.
Here we show that murine bone marrow derived macrophages differentiated in vitro with GM-CSF are also characterised by high levels of IRF5 mRNA and protein and express proinflammatory cytokines upon LPS stimulation.
These macrophages display characteristic expression of M1-marker MHC II but lack the M2-marker CD206.
Significantly, we develop intracellular staining of IRF5- expressing macrophages and utilise it to recapitulate the in vitro results in an in vivo model of antigen-induced arthritis, emphasising their physiological relevance.
Thus, we establish the species-invariant role of IRF5 in controlling the inflammatory macrophage phenotype both in vitro and in in vivo.
American Psychological Association (APA)
Weiss, Miriam& Blazek, Katrina& Byrne, Adam J.& Perocheau, Dany P.& Udalova, Irina A.. 2013. IRF5 Is a Specific Marker of Inflammatory Macrophages In Vivo. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-456940
Modern Language Association (MLA)
Weiss, Miriam…[et al.]. IRF5 Is a Specific Marker of Inflammatory Macrophages In Vivo. Mediators of Inflammation No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-456940
American Medical Association (AMA)
Weiss, Miriam& Blazek, Katrina& Byrne, Adam J.& Perocheau, Dany P.& Udalova, Irina A.. IRF5 Is a Specific Marker of Inflammatory Macrophages In Vivo. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-456940
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-456940