Cloning, Expression, Purification, and Characterization of Glutaredoxin from Antarctic Sea-Ice Bacterium Pseudoalteromonas sp. AN178
Joint Authors
Liu, Yuanping
Han, Xiao
Hu, Zhiguo
Hou, Yanhua
Shi, Yonglei
Chen, Qian
Wang, Quanfu
Li, YuJin
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-07
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Glutaredoxins (Grxs) are small ubiquitous redox enzymes that catalyze glutathione-dependent reactions to reduce protein disulfide.
In this study, a full-length Grx gene (PsGrx) with 270 nucleotides was isolated from Antarctic sea-ice bacterium Pseudoalteromonas sp.
AN178.
It encoded deduced 89 amino acid residues with the molecular weight 9.8 kDa.
Sequence analysis of the amino acid sequence revealed the catalytic motif CPYC.
Recombinant PsGrx (rPsGrx) stably expressed in E.
coli BL21 was purified to apparent homogeneity by Ni-affinity chromatography.
rPsGrx exhibited optimal activity at 30°C and pH 8.0 and showed 25.5% of the activity at 0°C.
It retained 65.0% of activity after incubation at 40°C for 20 min and still exhibited 37.0% activity in 1.0 M NaCl.
These results indicated that rPsGrx was a typical cold active protein with low thermostability.
American Psychological Association (APA)
Wang, Quanfu& Hou, Yanhua& Shi, Yonglei& Han, Xiao& Chen, Qian& Hu, Zhiguo…[et al.]. 2014. Cloning, Expression, Purification, and Characterization of Glutaredoxin from Antarctic Sea-Ice Bacterium Pseudoalteromonas sp. AN178. BioMed Research International،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-457036
Modern Language Association (MLA)
Wang, Quanfu…[et al.]. Cloning, Expression, Purification, and Characterization of Glutaredoxin from Antarctic Sea-Ice Bacterium Pseudoalteromonas sp. AN178. BioMed Research International No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-457036
American Medical Association (AMA)
Wang, Quanfu& Hou, Yanhua& Shi, Yonglei& Han, Xiao& Chen, Qian& Hu, Zhiguo…[et al.]. Cloning, Expression, Purification, and Characterization of Glutaredoxin from Antarctic Sea-Ice Bacterium Pseudoalteromonas sp. AN178. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-457036
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457036