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Electrochemical Immunoassay of Escherichia coli O157 : H7 Using Ag@SiO2 Nanoparticles as Labels
Joint Authors
Chen, Guang-Zhu
Lou, Jv-Feng
Yin, Zheng-Zhi
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Silica coated silver (Ag@SiO2) nanoparticles were prepared and characterized by transmission electron microscope (TEM) and UV-vis absorption, and the nanoparticles were used as labels in sandwich-type immunosensor of Escherichia coli O157:H7 (E.
coli O157:H7).
The labels involved in immunoreaction were dissolved by mixed acid of hydrofluoric acid and nitric acid, and the released Ag+ ions were electrochemical stripping analyzed (via differential pulse voltammetry, DPV) at poly(acrylic acid)/poly(diallyldimethylammonium chloride)/carbon nanotubes (PAA/PDCNT) modified glass carbon electrode (GCE), which obviously enhanced the signal of Ag+ stripping.
Then, the number of E.
coli O157:H7 could be indirectly reflected by the signal intensity of labeled Ag+.
And the results showed that the DPV signals were proportional to the logarithm of the E.
coli O157:H7 concentration in the range from 20 cfu/mL to 8.0×103 cfu/mL with the detection limit of 13 cfu/mL.
American Psychological Association (APA)
Chen, Guang-Zhu& Yin, Zheng-Zhi& Lou, Jv-Feng. 2014. Electrochemical Immunoassay of Escherichia coli O157 : H7 Using Ag@SiO2 Nanoparticles as Labels. Journal of Analytical Methods in Chemistry،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-457054
Modern Language Association (MLA)
Chen, Guang-Zhu…[et al.]. Electrochemical Immunoassay of Escherichia coli O157 : H7 Using Ag@SiO2 Nanoparticles as Labels. Journal of Analytical Methods in Chemistry No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-457054
American Medical Association (AMA)
Chen, Guang-Zhu& Yin, Zheng-Zhi& Lou, Jv-Feng. Electrochemical Immunoassay of Escherichia coli O157 : H7 Using Ag@SiO2 Nanoparticles as Labels. Journal of Analytical Methods in Chemistry. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-457054
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457054