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A Miniaturized Ligand Binding Assay for EGFR
Joint Authors
Schwenk, Jochen M.
Joos, Thomas O.
Poetz, Oliver
Zeillinger, Robert
Source
International Journal of Proteomics
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-08
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
In order to study receptor abundance and its function in solutions or in homogenates from clinical specimen, methods such as sandwich or radioimmunoassays are most commonly employed.
For the determination of epidermal growth factor receptor (EGFR), we describe the development of a miniaturized bead-based ligand binding assay using its ligand EGF as immobilized capture reagent.
This assay was used to analyze lysates from cell lines, and the ligand-bound EGFR was detected using an EGFR-specific antibody combined with a fluorescence-based reporter system.
In a proof-of concept study with lysates from breast biopsies, the assay allowed to classify breast cancer samples in accordance to clinically the relevant EGFR cut-off level.
The study suggests that such a ligand binding receptor assay could become an integral part of protein profiling procedures to provide additional information about receptor functionality in addition to its abundance.
American Psychological Association (APA)
Schwenk, Jochen M.& Poetz, Oliver& Zeillinger, Robert& Joos, Thomas O.. 2012. A Miniaturized Ligand Binding Assay for EGFR. International Journal of Proteomics،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-457059
Modern Language Association (MLA)
Schwenk, Jochen M.…[et al.]. A Miniaturized Ligand Binding Assay for EGFR. International Journal of Proteomics No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-457059
American Medical Association (AMA)
Schwenk, Jochen M.& Poetz, Oliver& Zeillinger, Robert& Joos, Thomas O.. A Miniaturized Ligand Binding Assay for EGFR. International Journal of Proteomics. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-457059
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457059