![](/images/graphics-bg.png)
Selective Binding of Distamycin A Derivative to G-Quadruplex Structure [d(TGGGGT)]4
Joint Authors
Giancola, Concetta
De Tito, Stefano
Pagano, Bruno
Randazzo, Antonio
Novellino, Ettore
Mattia, Carlo A.
Mayol, Luciano
Fotticchia, Iolanda
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-05-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Guanine-rich nucleic acid sequences can adopt G-quadruplex structures stabilized by layers of four Hoogsteen-paired guanine residues.
Quadruplex-prone sequences are found in many regions of human genome and in the telomeres of all eukaryotic organisms.
Since small molecules that target G-quadruplexes have been found to be effective telomerase inhibitors, the identification of new specific ligands for G-quadruplexes is emerging as a promising approach to develop new anticancer drugs.
Distamycin A is known to bind to AT-rich sequences of duplex DNA, but it has recently been shown to interact also with G-quadruplexes.
Here, isothermal titration calorimetry (ITC) and NMR techniques have been employed to characterize the interaction between a dicationic derivative of distamycin A (compound 1) and the [d(TGGGGT)]4 quadruplex.
Additionally, to compare the binding behaviour of netropsin and compound 1 to the same target, a calometric study of the interaction between netropsin and [d(TGGGGT)]4 has been performed.
Experiments show that netropsin and compound 1 are able to bind to [d(TGGGGT)]4 with good affinity and comparable thermodynamic profiles.
In both cases the interactions are entropically driven processes with a small favourable enthalpic contribution.
Interestingly, the structural modifications of compound 1 decrease the affinity of the ligand toward the duplex, enhancing the selectivity.
American Psychological Association (APA)
Pagano, Bruno& Fotticchia, Iolanda& De Tito, Stefano& Mattia, Carlo A.& Mayol, Luciano& Novellino, Ettore…[et al.]. 2010. Selective Binding of Distamycin A Derivative to G-Quadruplex Structure [d(TGGGGT)]4. Journal of Nucleic Acids،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-457066
Modern Language Association (MLA)
Pagano, Bruno…[et al.]. Selective Binding of Distamycin A Derivative to G-Quadruplex Structure [d(TGGGGT)]4. Journal of Nucleic Acids No. 2010 (2010), pp.1-7.
https://search.emarefa.net/detail/BIM-457066
American Medical Association (AMA)
Pagano, Bruno& Fotticchia, Iolanda& De Tito, Stefano& Mattia, Carlo A.& Mayol, Luciano& Novellino, Ettore…[et al.]. Selective Binding of Distamycin A Derivative to G-Quadruplex Structure [d(TGGGGT)]4. Journal of Nucleic Acids. 2010. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-457066
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457066