Peroxynitrite Mediates Diabetes-Induced Endothelial Dysfunction : Possible Role of Rho Kinase Activation
Joint Authors
Caldwell, R. William
Matragoon, Suraporn
Caldwell, Ruth B.
Pillai, Bindu
Tawfik, Huda E.
El-Remessy, Azza B.
Source
Experimental Diabetes Research
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-11-01
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Endothelial dysfunction is characterized by reduced bioavailability of NO due to its inactivation to form peroxynitrite or reduced expression of eNOS.
Here, we examine the causal role of peroxynitrite in mediating diabetes-induced endothelial dysfunction.
Diabetes was induced by STZ-injection, and rats received the peroxynitrite decomposition catalyst (FeTTPs, 15 mg/Kg/day) for 4 weeks.
Vasorelaxation to acetylcholine, oxidative-stress markers, RhoA activity, and eNOS expression were determined.
Diabetic coronary arteries showed significant reduction in ACh-mediated maximal relaxation compared to controls.
Diabetic vessels showed also significant increases in lipid-peroxides, nitrotyrosine, and active RhoA and 50% reduction in eNOS mRNA expression.
Treatment of diabetic animals with FeTTPS blocked these effects.
Studies in aortic endothelial cells show that high glucose or peroxynitrite increases the active RhoA kinase levels and decreases eNOS expression and NO levels, which were reversed with blocking peroxynitrite or Rho kinase.
Together, peroxynitrite can suppress eNOS expression via activation of RhoA and hence cause vascular dysfunction.
American Psychological Association (APA)
El-Remessy, Azza B.& Tawfik, Huda E.& Matragoon, Suraporn& Pillai, Bindu& Caldwell, Ruth B.& Caldwell, R. William. 2010. Peroxynitrite Mediates Diabetes-Induced Endothelial Dysfunction : Possible Role of Rho Kinase Activation. Experimental Diabetes Research،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-457136
Modern Language Association (MLA)
El-Remessy, Azza B.…[et al.]. Peroxynitrite Mediates Diabetes-Induced Endothelial Dysfunction : Possible Role of Rho Kinase Activation. Experimental Diabetes Research No. 2010 (2010), pp.1-9.
https://search.emarefa.net/detail/BIM-457136
American Medical Association (AMA)
El-Remessy, Azza B.& Tawfik, Huda E.& Matragoon, Suraporn& Pillai, Bindu& Caldwell, Ruth B.& Caldwell, R. William. Peroxynitrite Mediates Diabetes-Induced Endothelial Dysfunction : Possible Role of Rho Kinase Activation. Experimental Diabetes Research. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-457136
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457136