Neurotensin Decreases the Proinflammatory Status of Human Skin Fibroblasts and Increases Epidermal Growth Factor Expression
Joint Authors
Moura, Liane
Miguel Neves, Bruno
Cruz, Maria Teresa
Pereira da Silva, Lucília
Carvalho, Eugénia
Source
International Journal of Inflammation
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-08-11
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Fibroblasts colonization into injured areas during wound healing (WH) is responsible for skin remodelling and is also involved in the modulation of inflammation, as fibroblasts are immunologically active.
Herein, we aimed to determine neurotensin effect on the immunomodulatory profile of fibroblasts, both in homeostatic and inflammatory conditions.
Neurotensin mediated responses occurred through NTR1 or NTR3 receptors, while under inflammatory conditions NTR1 expression increase seemed to modulate neurotensin responses.
Among different immunomodulatory genes, CCL11, IL-8, and IL-6 were the most expressed genes, while CCL4 and EGF were the less expressed genes.
After neurotensin exposure, IL-8 mRNA expression was increased while CCL11 was decreased, suggesting a proinflammatory upregulation and chemoattractant ability downregulation of fibroblasts.
Under inflammatory conditions, gene expression was significantly increased.
After neurotensin exposure, CCL4 and IL-6 mRNA expression were decreased while CCL11 was increased, suggesting again a decrease in the chemoattractant capacity of fibroblasts and in their proinflammatory status.
Furthermore, the expression of EGF, a crucial growth factor for skin cells proliferation and WH, was increased in all conditions.
Overall, neurotensin, released by nerve fibers or skin cells, may be involved in the decrease of the chemotaxis and the proinflammatory status in the proliferation and remodelling phases of WH.
American Psychological Association (APA)
Pereira da Silva, Lucília& Miguel Neves, Bruno& Moura, Liane& Cruz, Maria Teresa& Carvalho, Eugénia. 2014. Neurotensin Decreases the Proinflammatory Status of Human Skin Fibroblasts and Increases Epidermal Growth Factor Expression. International Journal of Inflammation،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-457174
Modern Language Association (MLA)
Pereira da Silva, Lucília…[et al.]. Neurotensin Decreases the Proinflammatory Status of Human Skin Fibroblasts and Increases Epidermal Growth Factor Expression. International Journal of Inflammation No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-457174
American Medical Association (AMA)
Pereira da Silva, Lucília& Miguel Neves, Bruno& Moura, Liane& Cruz, Maria Teresa& Carvalho, Eugénia. Neurotensin Decreases the Proinflammatory Status of Human Skin Fibroblasts and Increases Epidermal Growth Factor Expression. International Journal of Inflammation. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-457174
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457174