Promoter Region Hypermethylation and mRNA Expression of MGMT and p16 Genes in Tissue and Blood Samples of Human Premalignant Oral Lesions and Oral Squamous Cell Carcinoma
Joint Authors
Bhatia, Vikram
Goel, Madhu Mati
Goel, Sudhir K.
Yadu, Alka
Tewari, Shikha
Shilpi, Priyanka
Agarwal, S. P.
Kumar, Sandeep
Makker, Annu
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-01
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Promoter methylation and relative gene expression of O6-methyguanine-DNA-methyltransferase (MGMT) and p16 genes were examined in tissue and blood samples of patients with premalignant oral lesions (PMOLs) and oral squamous cell carcinoma (OSCC).
Methylation-specific PCR and reverse transcriptase PCR were performed in 146 tissue and blood samples from controls and patients with PMOLs and OSCC.
In PMOL group, significant promoter methylation of MGMT and p16 genes was observed in 59% (P=0.0010) and 57% (P=0.0016) of tissue samples, respectively, and 39% (P=0.0135) and 33% (P=0.0074) of blood samples, respectively.
Promoter methylation of both genes was more frequent in patients with OSCC, that is, 76% (P=0.0001) and 82% (P=0.0001) in tissue and 57% (P=0.0002) and 70% (P=0.0001) in blood, respectively.
Significant downregulation of MGMT and p16 mRNA expression was observed in both tissue and blood samples from patients with PMOLs and OSCC.
Hypermethylation-induced transcriptional silencing of MGMT and p16 genes in both precancer and cancer suggests important role of these changes in progression of premalignant state to malignancy.
Results support use of blood as potential surrogate to tissue samples for screening or diagnosing PMOLs and early OSCC.
American Psychological Association (APA)
Bhatia, Vikram& Goel, Madhu Mati& Makker, Annu& Tewari, Shikha& Yadu, Alka& Shilpi, Priyanka…[et al.]. 2014. Promoter Region Hypermethylation and mRNA Expression of MGMT and p16 Genes in Tissue and Blood Samples of Human Premalignant Oral Lesions and Oral Squamous Cell Carcinoma. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-457195
Modern Language Association (MLA)
Bhatia, Vikram…[et al.]. Promoter Region Hypermethylation and mRNA Expression of MGMT and p16 Genes in Tissue and Blood Samples of Human Premalignant Oral Lesions and Oral Squamous Cell Carcinoma. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-457195
American Medical Association (AMA)
Bhatia, Vikram& Goel, Madhu Mati& Makker, Annu& Tewari, Shikha& Yadu, Alka& Shilpi, Priyanka…[et al.]. Promoter Region Hypermethylation and mRNA Expression of MGMT and p16 Genes in Tissue and Blood Samples of Human Premalignant Oral Lesions and Oral Squamous Cell Carcinoma. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-457195
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457195