Isolation, Purification, and Characterization of Fungal Laccase from Pleurotus sp.
Joint Authors
S. M., Veena
P. S., Renuka
Malini, S.
Pruthvi K.
Swetha M.
More, Sunil S.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-29
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Laccases are blue copper oxidases (E.C.
1.10.3.2 benzenediol: oxygen oxidoreductase) that catalyze the one-electron oxidation of phenolics, aromatic amines, and other electron-rich substrates with the concomitant reduction of O2 to H2O.
They are currently seen as highly interesting industrial enzymes because of their broad substrate specificity.
A positive strain was isolated and characterized as nonspore forming Basidiomycetes Pleurotus sp.
Laccase activity was determined using ABTS as substrate.
Laccase was purified by ionexchange and gel filtration chromatography.
The purified laccase was a monomer showed a molecular mass of 40±1 kDa as estimated by SDS-PAGE and a 72-fold purification with a 22% yield.
The optimal pH and temperature were 4.5 and 65°C, respectively.
The Km and Vmax values are 250 (mM) and 0.33 (μmol/min), respectively, for ABTS as substrate.
Metal ions like CuSO4, BaCl2, MgCl2, FeCl2, ZnCl2 have no effect on purified laccase whereas HgCl2 and MnCl2 moderately decrease enzyme activity.
SDS and sodium azide inhibited enzyme activity, whereas Urea, PCMB, DTT, and mercaptoethanol have no effect on enzyme activity.
The isolated laccase can be used in development of biosensor for detecting the phenolic compounds from the effluents of paper industries.
American Psychological Association (APA)
More, Sunil S.& P. S., Renuka& Pruthvi K.& Swetha M.& Malini, S.& S. M., Veena. 2011. Isolation, Purification, and Characterization of Fungal Laccase from Pleurotus sp.. Enzyme Research،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-457228
Modern Language Association (MLA)
More, Sunil S.…[et al.]. Isolation, Purification, and Characterization of Fungal Laccase from Pleurotus sp.. Enzyme Research No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-457228
American Medical Association (AMA)
More, Sunil S.& P. S., Renuka& Pruthvi K.& Swetha M.& Malini, S.& S. M., Veena. Isolation, Purification, and Characterization of Fungal Laccase from Pleurotus sp.. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-457228
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457228