Evaluation of Bax and Bak Gene Mutations and Expression in Breast Cancer
Joint Authors
El-Kased, Ahmed F.
Kholoussi, Naglaa Mohamed
Abd El-Bary, Naser Mohamed
Esmaiel, Nora Nassef
El-Nabi, Sobhy E. H.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-09
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Genetic analyses have provided evidence to suggest that Bax and Bak are the essential genes for apoptosis in mammalians cells.
This study aimed to search for biomarkers in breast cancer to be used as prognostic markers for the disease.
The Bak and Bax genes expressions were analyzed in 23 breast cancer patients by RT-PCR technique.
SSCP technique was used to detect the mobility of the abnormal fragment in Bak exon 4.
PCR for Bax promoter was digested with Tau 1 restriction enzyme to identify a single polymorphism G(-248)A.
The expression of Bak gene is related to several clinical factors of breast cancer.
The analysis of Bax RNA showed 4 isoforms of Bax with different distributions in the normal and tumor tissues.
These isoforms were Bax α, d, δ, and ζ.
Exon 4 had a normal pattern in all cases of breast cancer.
There was a statistically significant difference in the frequency distribution of the G(-248)A genotypes in the breast cancer tissues with grade 3+high, T2 stage, lobular +other, and PR −ve subgroups.
In this study, Bak expression seems to lead to development of breast cancer and affects the disease progression.
Also, Bax d and Bax δ could be used as risk factor and biomarker for breast cancer with the distribution of G284A.
American Psychological Association (APA)
Kholoussi, Naglaa Mohamed& El-Nabi, Sobhy E. H.& Esmaiel, Nora Nassef& Abd El-Bary, Naser Mohamed& El-Kased, Ahmed F.. 2014. Evaluation of Bax and Bak Gene Mutations and Expression in Breast Cancer. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-457291
Modern Language Association (MLA)
Kholoussi, Naglaa Mohamed…[et al.]. Evaluation of Bax and Bak Gene Mutations and Expression in Breast Cancer. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-457291
American Medical Association (AMA)
Kholoussi, Naglaa Mohamed& El-Nabi, Sobhy E. H.& Esmaiel, Nora Nassef& Abd El-Bary, Naser Mohamed& El-Kased, Ahmed F.. Evaluation of Bax and Bak Gene Mutations and Expression in Breast Cancer. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-457291
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457291