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Demonstration of Hepatitis C Virus RNA with In Situ Hybridization Employing a Locked Nucleic Acid Probe in Humanized Liver of Infected Chimeric Mice and in Needle-Biopsied Human Liver
Joint Authors
Kohara, Michinori
Onouchi, Takanori
Shiogama, Kazuya
Inada, Ken-ichi
Teramoto, Hidemi
Mizutani, Yasuyoshi
Tsutsumi, Yutaka
Source
International Journal of Hepatology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-18
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
In situ hybridization (ISH) with high sensitivity has been requested to demonstrate hepatitis C virus (HCV) RNA in formalin-fixed, paraffin-embedded (FFPE) sections of the liver.
Methods.
ISH employing a locked-nucleic-acid- (LNA-)modified oligonucleotide probe and biotin-free catalyzed signal amplification system (CSAII) was applied to HCV-RNA detection in the liver tissue.
Nested reverse-transcription polymerase chain reaction (RT-PCR) was performed for HCV genotyping using total RNA extracted from FFPE sections.
The target tissues included FFPE tissue sections of humanized livers in HCV-infected chimeric mice (HCV genotypes 1a, 1b, and 2a and noninfected) and of needle-biopsied livers from HCV-infected patients.
Results.
HCV-RNA was demonstrated with the ISH technique in HCV-infected liver tissues from both chimeric mice and 9 (82%) of 11 patients with HCV infection.
The HCV signals were sensitive to RNase.
Nested RT-PCR confirmed the genotype in 8 (73%) of 11 livers (type 1b: 6 lesions and type 2a: 2 lesions).
HCV-RNA was not identified in chronic hepatitis B lesions, fatty liver, autoimmune hepatitis, and hepatocellular carcinoma.
Conclusion.
ISH using the LNA-modified oligonucleotide probe and CSAII was applicable to detecting HCV-RNA in routinely prepared FFPE liver specimens.
American Psychological Association (APA)
Shiogama, Kazuya& Inada, Ken-ichi& Kohara, Michinori& Teramoto, Hidemi& Mizutani, Yasuyoshi& Onouchi, Takanori…[et al.]. 2013. Demonstration of Hepatitis C Virus RNA with In Situ Hybridization Employing a Locked Nucleic Acid Probe in Humanized Liver of Infected Chimeric Mice and in Needle-Biopsied Human Liver. International Journal of Hepatology،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-457313
Modern Language Association (MLA)
Shiogama, Kazuya…[et al.]. Demonstration of Hepatitis C Virus RNA with In Situ Hybridization Employing a Locked Nucleic Acid Probe in Humanized Liver of Infected Chimeric Mice and in Needle-Biopsied Human Liver. International Journal of Hepatology No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-457313
American Medical Association (AMA)
Shiogama, Kazuya& Inada, Ken-ichi& Kohara, Michinori& Teramoto, Hidemi& Mizutani, Yasuyoshi& Onouchi, Takanori…[et al.]. Demonstration of Hepatitis C Virus RNA with In Situ Hybridization Employing a Locked Nucleic Acid Probe in Humanized Liver of Infected Chimeric Mice and in Needle-Biopsied Human Liver. International Journal of Hepatology. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-457313
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457313