The In Vitro Susceptibility of Biofilm Forming Medical Device Related Pathogens to Conventional Antibiotics
Joint Authors
Laverty, Garry
Gilmore, Brendan F.
Alkawareek, Mahmoud Y.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-01
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and minimum biofilm eradication concentration (MBEC) and kill kinetics were established for vancomycin, rifampicin, trimethoprim, gentamicin, and ciprofloxacin against the biofilm forming bacteria Staphylococcus epidermidis (ATCC 35984), Staphylococcus aureus (ATCC 29213), Methicillin Resistant Staphylococcus aureus (MRSA) (ATCC 43300), Pseudomonas aeruginosa (PAO1), and Escherichia coli (NCTC 8196).
MICs and MBCs were determined via broth microdilution in 96-well plates.
MBECs were studied using the Calgary Biofilm Device.
Values obtained were used to investigate the kill kinetics of conventional antimicrobials against a range of planktonic and biofilm microorganisms over a period of 24 hours.
Planktonic kill kinetics were determined at 4xMIC and biofilm kill kinetics at relative MBECs.
Susceptibility of microorganisms varied depending on antibiotic selected and phenotypic form of bacteria.
Gram-positive planktonic isolates were extremely susceptible to vancomycin (highest MBC: 7.81 mg L−1: methicillin sensitive and resistant S.
aureus) but no MBEC value was obtained against all biofilm pathogens tested (up to 1000 mg L−1).
Both gentamicin and ciprofloxacin displayed the broadest spectrum of activity with MIC and MBCs in the mg L−1 range against all planktonic isolates tested and MBEC values obtained against all but S.
epidermidis (ATCC 35984) and MRSA (ATCC 43300).
American Psychological Association (APA)
Laverty, Garry& Alkawareek, Mahmoud Y.& Gilmore, Brendan F.. 2014. The In Vitro Susceptibility of Biofilm Forming Medical Device Related Pathogens to Conventional Antibiotics. Dataset Papers in Science،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-457413
Modern Language Association (MLA)
Laverty, Garry…[et al.]. The In Vitro Susceptibility of Biofilm Forming Medical Device Related Pathogens to Conventional Antibiotics. Dataset Papers in Science No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-457413
American Medical Association (AMA)
Laverty, Garry& Alkawareek, Mahmoud Y.& Gilmore, Brendan F.. The In Vitro Susceptibility of Biofilm Forming Medical Device Related Pathogens to Conventional Antibiotics. Dataset Papers in Science. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-457413
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457413