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Blockade of Airway Inflammation by Kaempferol via Disturbing Tyk-STAT Signaling in Airway Epithelial Cells and in Asthmatic Mice
Joint Authors
Kang, Young-Hee
Han, Seon-Young
Kang, Min-Kyung
Kim, Jung-Lye
Park, Sin-Hye
Shin, Daekeun
Gong, Ju-Hyun
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-08
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Asthma is characterized by bronchial inflammation causing increased airway hyperresponsiveness and eosinophilia.
The interaction between airway epithelium and inflammatory mediators plays a key role in the asthmatic pathogenesis.
The in vitro study elucidated inhibitory effects of kaempferol, a flavonoid found in apples and many berries, on inflammation in human airway epithelial BEAS-2B cells.
Nontoxic kaempferol at ≤20 μM suppressed the LPS-induced IL-8 production through the TLR4 activation, inhibiting eotaxin-1 induction.
The in vivo study explored the demoting effects of kaempferol on asthmatic inflammation in BALB/c mice sensitized with ovalbumin (OVA).
Mouse macrophage inflammatory protein-2 production and CXCR2 expression were upregulated in OVA-challenged mice, which was attenuated by oral administration of ≥10 mg/kg kaempferol.
Kaempferol allayed the airway tissue levels of eotaxin-1 and eotaxin receptor CCR3 enhanced by OVA challenge.
This study further explored the blockade of Tyk-STAT signaling by kaempferol in both LPS-stimulated BEAS-2B cells and OVA-challenged mice.
LPS activated Tyk2 responsible for eotaxin-1 induction, while kaempferol dose-dependently inhibited LPS- or IL-8-inflamed Tyk2 activation.
Similar inhibition of Tyk2 activation by kaempferol was observed in OVA-induced mice.
Additionally, LPS stimulated the activation of STAT1/3 signaling concomitant with downregulated expression of Tyk-inhibiting SOCS3.
In contrast, kaempferol encumbered STAT1/3 signaling with restoration of SOCS3 expression.
Consistently, oral administration of kaempferol blocked STAT3 transactivation elevated by OVA challenge.
These results demonstrate that kaempferol alleviated airway inflammation through modulating Tyk2-STAT1/3 signaling responsive to IL-8 in endotoxin-exposed airway epithelium and in asthmatic mice.
Therefore, kaempferol may be a therapeutic agent targeting asthmatic diseases.
American Psychological Association (APA)
Gong, Ju-Hyun& Shin, Daekeun& Han, Seon-Young& Park, Sin-Hye& Kang, Min-Kyung& Kim, Jung-Lye…[et al.]. 2013. Blockade of Airway Inflammation by Kaempferol via Disturbing Tyk-STAT Signaling in Airway Epithelial Cells and in Asthmatic Mice. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-457418
Modern Language Association (MLA)
Gong, Ju-Hyun…[et al.]. Blockade of Airway Inflammation by Kaempferol via Disturbing Tyk-STAT Signaling in Airway Epithelial Cells and in Asthmatic Mice. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-457418
American Medical Association (AMA)
Gong, Ju-Hyun& Shin, Daekeun& Han, Seon-Young& Park, Sin-Hye& Kang, Min-Kyung& Kim, Jung-Lye…[et al.]. Blockade of Airway Inflammation by Kaempferol via Disturbing Tyk-STAT Signaling in Airway Epithelial Cells and in Asthmatic Mice. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-457418
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457418