Histological and Immunohistochemical Evaluation of Autologous Cultured Bone Marrow Mesenchymal Stem Cells and Bone Marrow Mononucleated Cells in Collagenase-Induced Tendinitis of Equine Superficial Digital Flexor Tendon
Joint Authors
Lacitignola, Luca
Crovace, Antonio
Francioso, Edda
Rossi, Giacomo
Source
Veterinary Medicine International
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-03-22
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The aim of this study was to compare treatment with cultured bone marrow stromal cells (cBMSCs), bone marrow Mononucleated Cells (BMMNCs), and placebo to repair collagenase-induced tendinitis in horses.
In six adult Standardbred horses, 4000 IU of collagenase were injected in the superficial digital flexor tendon (SDFT).
Three weeks after collagenase treatment, an average of either 5.5×106 cBMSCs or 1.2×108 BMMNCs, fibrin glue, and saline solution was injected intralesionally in random order.
In cBMSC- and BMMNCS-treated tendons, a high expression of cartilage oligomeric matrix protein (COMP) and type I collagen, but low levels of type III collagen were revealed by immunohistochemistry, with a normal longitudinally oriented fiber pattern.
Placebo-treated tendons expressed very low quantities of COMP and type I collagen but large numbers of randomly oriented type III collagen fibers.
Both cBMSC and BMMNCS grafts resulted in a qualitatively similar heling improvement of tendon extracellular matrix, in terms of the type I/III collagen ratio, fiber orientation, and COMP expression.
American Psychological Association (APA)
Crovace, Antonio& Lacitignola, Luca& Rossi, Giacomo& Francioso, Edda. 2010. Histological and Immunohistochemical Evaluation of Autologous Cultured Bone Marrow Mesenchymal Stem Cells and Bone Marrow Mononucleated Cells in Collagenase-Induced Tendinitis of Equine Superficial Digital Flexor Tendon. Veterinary Medicine International،Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-457451
Modern Language Association (MLA)
Crovace, Antonio…[et al.]. Histological and Immunohistochemical Evaluation of Autologous Cultured Bone Marrow Mesenchymal Stem Cells and Bone Marrow Mononucleated Cells in Collagenase-Induced Tendinitis of Equine Superficial Digital Flexor Tendon. Veterinary Medicine International No. 2010 (2010), pp.1-10.
https://search.emarefa.net/detail/BIM-457451
American Medical Association (AMA)
Crovace, Antonio& Lacitignola, Luca& Rossi, Giacomo& Francioso, Edda. Histological and Immunohistochemical Evaluation of Autologous Cultured Bone Marrow Mesenchymal Stem Cells and Bone Marrow Mononucleated Cells in Collagenase-Induced Tendinitis of Equine Superficial Digital Flexor Tendon. Veterinary Medicine International. 2010. Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-457451
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457451