Inhibition of Cell Growth and Cellular Protein, DNA and RNA Synthesis in Human Hepatoma (HepG2) Cells by Ethanol Extract of Abnormal Savda Munziq of Traditional Uighur Medicine
Joint Authors
Yusup, Abdiryim
Yimit, Dilxat
Moore, Nicholas
Lapham, Jaya Conser
Halmurati, Upur
Creppy, Edmon E.
Berke, Benedicte
Umar, Anwar
Baudrimont, Isabelle
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-06-18
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Abnormal Savda Munziq (ASMq) is a traditional Uighur medicinal herbal preparation, commonly used for the treatment and prevention of cancer.
We tested the effects of ethanol extract of ASMq on cultured human hepatoma cells (HepG2) to explore the mechanism of its putative anticancer properties, using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) bromide, neutral red and lactate dehydrogenase (LDH) leakage assays, testing the incorporation of 3[H]-leucine and 3[H]-nucleosides into protein, DNA and RNA, and quantifying the formation of malondialdehyde-thiobarbituric acid (MDA) adducts.
ASMq ethanol extract significantly inhibited the growth of HepG2 and cell viability, increased the leakage of LDH after 48 hours or 72 hours treatment, in a concentration- and time-dependent manner (P < .05).
Cellular protein, DNA and RNA synthesis were inhibited in a concentration- and time-dependent manner (P < .05).
No significant MDA release in culture medium and no lipid peroxidation in cells were observed.
The results suggest that the cytotoxic effects of ASMq ethanol extract might be related to inhibition of cancer cell growth, alteration of cell membrane integrity and inhibition of cellular protein, DNA and RNA synthesis.
American Psychological Association (APA)
Halmurati, Upur& Yusup, Abdiryim& Baudrimont, Isabelle& Umar, Anwar& Berke, Benedicte& Yimit, Dilxat…[et al.]. 2011. Inhibition of Cell Growth and Cellular Protein, DNA and RNA Synthesis in Human Hepatoma (HepG2) Cells by Ethanol Extract of Abnormal Savda Munziq of Traditional Uighur Medicine. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-457482
Modern Language Association (MLA)
Halmurati, Upur…[et al.]. Inhibition of Cell Growth and Cellular Protein, DNA and RNA Synthesis in Human Hepatoma (HepG2) Cells by Ethanol Extract of Abnormal Savda Munziq of Traditional Uighur Medicine. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-457482
American Medical Association (AMA)
Halmurati, Upur& Yusup, Abdiryim& Baudrimont, Isabelle& Umar, Anwar& Berke, Benedicte& Yimit, Dilxat…[et al.]. Inhibition of Cell Growth and Cellular Protein, DNA and RNA Synthesis in Human Hepatoma (HepG2) Cells by Ethanol Extract of Abnormal Savda Munziq of Traditional Uighur Medicine. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-9.
https://search.emarefa.net/detail/BIM-457482
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457482