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Assessment of DNA Damage by RAPD in Paracentrotus lividus Embryos Exposed to Amniotic Fluid from Residents Living Close to Waste Landfill Sites
Joint Authors
Di Spiezio Sardo, Attilio
Ferrara, Cinzia
Bifulco, Giuseppe
Nappi, Carmine
Guida, Maurizio
D'Alessandro, Raffaella
Scognamiglio, Marianna
Santafede, Daniela
De Felice, Bruna
Guida, Marco
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-07-11
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The aim of this study was to assess the genotoxic effects of environmental chemicals on residents living near landfills.
The study was based on samples of amniotic fluid from women living in the intensely polluted areas around the Campania region of Italy compared to a nonexposed control group.
We evaluated the genetic effects that this amniotic fluids collected in contaminated sites had on Paracentrotus lividus embryos.
DNA damage was detected through changes in RAPD (Random Amplified Polymorphism DNA) profiles.
The absence of the amplified DNA fragments indicated deletions in Paracentrotus lividus DNA exposed to the contaminated amniotic fluids when compared to equal exposure to uncontaminated fluids.
These results show the ability of RAPD-PCR to detect and isolate DNA sequences representing genetic alterations induced in P.
lividus embryos.
Using this method, we identified two candidate target regions for DNA alterations in the genome of P.
lividus.
Our research indicates that RAPD-PCR in P.
lividus embryo DNA can provide a molecular approach for studying DNA damage from pollutants that can impact human health.
To our knowledge, this is the first time that assessment of DNA damage in P.
lividus embryos has been tested using the RAPD strategy after exposure to amniotic fluid from residents near waste landfill sites.
American Psychological Association (APA)
Guida, Maurizio& Guida, Marco& De Felice, Bruna& Santafede, Daniela& D'Alessandro, Raffaella& Di Spiezio Sardo, Attilio…[et al.]. 2010. Assessment of DNA Damage by RAPD in Paracentrotus lividus Embryos Exposed to Amniotic Fluid from Residents Living Close to Waste Landfill Sites. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-457535
Modern Language Association (MLA)
Guida, Maurizio…[et al.]. Assessment of DNA Damage by RAPD in Paracentrotus lividus Embryos Exposed to Amniotic Fluid from Residents Living Close to Waste Landfill Sites. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-7.
https://search.emarefa.net/detail/BIM-457535
American Medical Association (AMA)
Guida, Maurizio& Guida, Marco& De Felice, Bruna& Santafede, Daniela& D'Alessandro, Raffaella& Di Spiezio Sardo, Attilio…[et al.]. Assessment of DNA Damage by RAPD in Paracentrotus lividus Embryos Exposed to Amniotic Fluid from Residents Living Close to Waste Landfill Sites. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-457535
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457535