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Detection of EpCAM-Negative and Cytokeratin-Negative Circulating Tumor Cells in Peripheral Blood
Joint Authors
Mikolajczyk, Stephen D.
Tsinberg, Pavel
Zomorrodi, Maryam
Bischoff, Farideh Z.
Pircher, Tony J.
Pham, Tam
Millar, Lisa S.
Coutts, Stephen M.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-04-19
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Enrichment of rare circulating tumor cells (CTCs) in blood is typically achieved using antibodies to epithelial cell adhesion molecule (EpCAM), with detection using cytokeratin (CK) antibodies.
However, EpCAM and CK are not expressed in some tumors and can be downregulated during epithelial-to-mesenchymal transition.
A micro-fluidic system, not limited to EpCAM or CK, was developed to use multiple antibodies for capture followed by detection using CEE-Enhanced (CE), a novel in situ staining method that fluorescently labels the capture antibodies bound to CTCs.
Higher recovery of CTCs was demonstrated using antibody mixtures compared to anti-EpCAM.
In addition, CK-positive breast cancer cells were found in 15 of 24 samples (63%; range 1–60 CTCs), while all samples contained additional CE-positive cells (range 1–41; median = 11; P=.02).
Thus, antibody mixtures against a range of cell surface antigens enables capture of more CTCs than anti-EpCAM alone and CE staining enables the detection of CK-negative CTCs.
American Psychological Association (APA)
Mikolajczyk, Stephen D.& Millar, Lisa S.& Tsinberg, Pavel& Coutts, Stephen M.& Zomorrodi, Maryam& Pham, Tam…[et al.]. 2011. Detection of EpCAM-Negative and Cytokeratin-Negative Circulating Tumor Cells in Peripheral Blood. Journal of Oncology،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-457574
Modern Language Association (MLA)
Mikolajczyk, Stephen D.…[et al.]. Detection of EpCAM-Negative and Cytokeratin-Negative Circulating Tumor Cells in Peripheral Blood. Journal of Oncology No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-457574
American Medical Association (AMA)
Mikolajczyk, Stephen D.& Millar, Lisa S.& Tsinberg, Pavel& Coutts, Stephen M.& Zomorrodi, Maryam& Pham, Tam…[et al.]. Detection of EpCAM-Negative and Cytokeratin-Negative Circulating Tumor Cells in Peripheral Blood. Journal of Oncology. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-457574
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457574