Systemic siRNA Delivery via Peptide-Tagged Polymeric Nanoparticles, Targeting PLK1 Gene in a Mouse Xenograft Model of Colorectal Cancer
Joint Authors
Malhotra, Meenakshi
Tomaro-Duchesneau, Catherine
Prakash, Satya
Saha, Shyamali
Source
International Journal of Biomaterials
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-15
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Polymeric nanoparticles were developed from a series of chemical reactions using chitosan, polyethylene glycol, and a cell-targeting peptide (CP15).
The nanoparticles were complexed with PLK1-siRNA.
The optimal siRNA loading was achieved at an N : P ratio of 129.2 yielding a nanoparticle size of >200 nm.
These nanoparticles were delivered intraperitoneally and tested for efficient delivery, cytotoxicity, and biodistribution in a mouse xenograft model of colorectal cancer.
Both unmodified and modified chitosan nanoparticles showed enhanced accumulation at the tumor site.
However, the modified chitosan nanoparticles showed considerably, less distribution in other organs.
The relative gene expression as evaluated showed efficient delivery of PLK1-siRNA (0.5 mg/kg) with 50.7±19.5% knockdown (P=0.031) of PLK1 gene.
The in vivo data reveals no systemic toxicity in the animals, when tested for systemic inflammation and liver toxicity.
These results indicate a potential of using peptide-tagged nanoparticles for systemic delivery of siRNA at the targeted tumor site.
American Psychological Association (APA)
Malhotra, Meenakshi& Tomaro-Duchesneau, Catherine& Saha, Shyamali& Prakash, Satya. 2013. Systemic siRNA Delivery via Peptide-Tagged Polymeric Nanoparticles, Targeting PLK1 Gene in a Mouse Xenograft Model of Colorectal Cancer. International Journal of Biomaterials،Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-457587
Modern Language Association (MLA)
Malhotra, Meenakshi…[et al.]. Systemic siRNA Delivery via Peptide-Tagged Polymeric Nanoparticles, Targeting PLK1 Gene in a Mouse Xenograft Model of Colorectal Cancer. International Journal of Biomaterials No. 2013 (2013), pp.1-13.
https://search.emarefa.net/detail/BIM-457587
American Medical Association (AMA)
Malhotra, Meenakshi& Tomaro-Duchesneau, Catherine& Saha, Shyamali& Prakash, Satya. Systemic siRNA Delivery via Peptide-Tagged Polymeric Nanoparticles, Targeting PLK1 Gene in a Mouse Xenograft Model of Colorectal Cancer. International Journal of Biomaterials. 2013. Vol. 2013, no. 2013, pp.1-13.
https://search.emarefa.net/detail/BIM-457587
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457587