Application of HPLC for the Simultaneous Determination of Paracetamol, Chlorzoxazone, and Nimesulide in Pharmaceutical Dosage Form
Joint Authors
Nikam, Ajinkya R.
Mahadik, Kakasaheb R.
More, Snehal J.
Tandulwadkar, Suparna S.
Rathore, Atul S.
Sathiyanarayanan, L.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-11
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A simple, precise, and accurate reversed-phase liquid chromatographic method has been developed for the simultaneous determination of paracetamol (PCM), chlorzoxazone (CHZ), and nimesulide (NIM) in pharmaceutical dosage form.
The chromatographic separation was achieved on a Thermo Hypersil GOLD C18 column (250 × 4.6 mm i.d., 5 μm particle size).
The mobile phase consisted of water : acetonitrile (55 : 45 v/v).
The flow rate was set to 1.2 mL min−1 and UV detection was carried out at 275 nm.
The retention time (tR) for PCM, CHZ, and NIM was found to be 2.69 ± 0.02, 4.61 ± 0.01, and 9.55 ± 0.02 min, respectively.
The validation of the proposed method was carried out for linearity, precision, robustness, limit of detection, limit of quantitation, specificity, and accuracy.
The linear dynamic ranges were 32.5–65.0 μg mL−1 for PCM, 37.5–75.0 μg mL−1 for CHZ, and 10.0–20.0 μg mL−1 for NIM.
The developed method can be used for routine quality control analysis of titled drugs in pharmaceutical dosage form.
American Psychological Association (APA)
More, Snehal J.& Tandulwadkar, Suparna S.& Nikam, Ajinkya R.& Rathore, Atul S.& Sathiyanarayanan, L.& Mahadik, Kakasaheb R.. 2012. Application of HPLC for the Simultaneous Determination of Paracetamol, Chlorzoxazone, and Nimesulide in Pharmaceutical Dosage Form. ISRN Chromatography،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-457614
Modern Language Association (MLA)
More, Snehal J.…[et al.]. Application of HPLC for the Simultaneous Determination of Paracetamol, Chlorzoxazone, and Nimesulide in Pharmaceutical Dosage Form. ISRN Chromatography No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-457614
American Medical Association (AMA)
More, Snehal J.& Tandulwadkar, Suparna S.& Nikam, Ajinkya R.& Rathore, Atul S.& Sathiyanarayanan, L.& Mahadik, Kakasaheb R.. Application of HPLC for the Simultaneous Determination of Paracetamol, Chlorzoxazone, and Nimesulide in Pharmaceutical Dosage Form. ISRN Chromatography. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-457614
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-457614